Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 481-484 |
| Seitenumfang | 4 |
| Fachzeitschrift | Biotechnology Techniques |
| Jahrgang | 10 |
| Ausgabenummer | 7 |
| Publikationsstatus | Veröffentlicht - 1996 |
| Extern publiziert | Ja |
Abstract
The efficiency of purification of basic fibroblast growth factor (bFGF) inclusion bodies using EDTA and nonionic detergents was improved from 25 to 40% by shifting the pH from 8.5 to strong alkaline conditions (pH 9.5 - 10.5). Complete dissolution of bFGF inclusion bodies by guanidinium hydrochloride (> 3 M) was independent of pH and the presence of reducing agents. In contrast, solubilization of bFCF inclusion bodies by urea was pH-dependent and increased in efficiency (e.g. from 0 to 100%) by increasing the pH (from pH 5.0 to 10.5 at 9 M urea). The purification and solubilization procedures are efficient for inclusion body concentrations corresponding to 10 and 100 g per I dry cell weight, respectively.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
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in: Biotechnology Techniques, Jahrgang 10, Nr. 7, 1996, S. 481-484.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Optimized procedures for purification and solubilization of basic fibroblast growth factor inclusion bodies
AU - Estapé, D.
AU - Rinas, U.
PY - 1996
Y1 - 1996
N2 - The efficiency of purification of basic fibroblast growth factor (bFGF) inclusion bodies using EDTA and nonionic detergents was improved from 25 to 40% by shifting the pH from 8.5 to strong alkaline conditions (pH 9.5 - 10.5). Complete dissolution of bFGF inclusion bodies by guanidinium hydrochloride (> 3 M) was independent of pH and the presence of reducing agents. In contrast, solubilization of bFCF inclusion bodies by urea was pH-dependent and increased in efficiency (e.g. from 0 to 100%) by increasing the pH (from pH 5.0 to 10.5 at 9 M urea). The purification and solubilization procedures are efficient for inclusion body concentrations corresponding to 10 and 100 g per I dry cell weight, respectively.
AB - The efficiency of purification of basic fibroblast growth factor (bFGF) inclusion bodies using EDTA and nonionic detergents was improved from 25 to 40% by shifting the pH from 8.5 to strong alkaline conditions (pH 9.5 - 10.5). Complete dissolution of bFGF inclusion bodies by guanidinium hydrochloride (> 3 M) was independent of pH and the presence of reducing agents. In contrast, solubilization of bFCF inclusion bodies by urea was pH-dependent and increased in efficiency (e.g. from 0 to 100%) by increasing the pH (from pH 5.0 to 10.5 at 9 M urea). The purification and solubilization procedures are efficient for inclusion body concentrations corresponding to 10 and 100 g per I dry cell weight, respectively.
UR - http://www.scopus.com/inward/record.url?scp=3542997762&partnerID=8YFLogxK
U2 - 10.1007/BF00159510
DO - 10.1007/BF00159510
M3 - Article
AN - SCOPUS:3542997762
VL - 10
SP - 481
EP - 484
JO - Biotechnology Techniques
JF - Biotechnology Techniques
SN - 0951-208X
IS - 7
ER -