Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 47 |
| Fachzeitschrift | Biology |
| Jahrgang | 5 |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - 30 Nov. 2016 |
Abstract
The application of DNA microarrays for high throughput analysis of genetic regulation is often limited by the fluorophores used as markers. The implementation of multi-scan techniques is limited by the fluorophores’ susceptibility to photobleaching when exposed to the scanner laser light. This paper presents combined mechanical and chemical strategies which enhance the photostability of cyanine 3 and cyanine 5 as part of solid state DNA microarrays. These strategies are based on scanning the microarrays while the hybridized DNA is still in an aqueous solution with the presence of a reductive/oxidative system (ROXS). Furthermore, the experimental setup allows for the analysis and eventual normalization of Förster-resonance-energy-transfer (FRET) interaction of cyanine-3/cyanine-5 dye combinations on the microarray. These findings constitute a step towards standardization of microarray experiments and analysis and may help to increase the comparability of microarray experiment results between labs.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Allgemeine Biochemie, Genetik und Molekularbiologie
- Immunologie und Mikrobiologie (insg.)
- Allgemeine Immunologie und Mikrobiologie
- Agrar- und Biowissenschaften (insg.)
- Allgemeine Agrar- und Biowissenschaften
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in: Biology, Jahrgang 5, Nr. 4, 47, 30.11.2016.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Optimization of Cyanine Dye Stability and Analysis of FRET Interaction on DNA Microarrays
AU - von der Haar, Marcel
AU - Heuer, Christopher
AU - Pähler, Martin
AU - von der Haar, Kathrin
AU - Lindner, Patrick
AU - Scheper, Thomas
AU - Stahl, Frank
PY - 2016/11/30
Y1 - 2016/11/30
N2 - The application of DNA microarrays for high throughput analysis of genetic regulation is often limited by the fluorophores used as markers. The implementation of multi-scan techniques is limited by the fluorophores’ susceptibility to photobleaching when exposed to the scanner laser light. This paper presents combined mechanical and chemical strategies which enhance the photostability of cyanine 3 and cyanine 5 as part of solid state DNA microarrays. These strategies are based on scanning the microarrays while the hybridized DNA is still in an aqueous solution with the presence of a reductive/oxidative system (ROXS). Furthermore, the experimental setup allows for the analysis and eventual normalization of Förster-resonance-energy-transfer (FRET) interaction of cyanine-3/cyanine-5 dye combinations on the microarray. These findings constitute a step towards standardization of microarray experiments and analysis and may help to increase the comparability of microarray experiment results between labs.
AB - The application of DNA microarrays for high throughput analysis of genetic regulation is often limited by the fluorophores used as markers. The implementation of multi-scan techniques is limited by the fluorophores’ susceptibility to photobleaching when exposed to the scanner laser light. This paper presents combined mechanical and chemical strategies which enhance the photostability of cyanine 3 and cyanine 5 as part of solid state DNA microarrays. These strategies are based on scanning the microarrays while the hybridized DNA is still in an aqueous solution with the presence of a reductive/oxidative system (ROXS). Furthermore, the experimental setup allows for the analysis and eventual normalization of Förster-resonance-energy-transfer (FRET) interaction of cyanine-3/cyanine-5 dye combinations on the microarray. These findings constitute a step towards standardization of microarray experiments and analysis and may help to increase the comparability of microarray experiment results between labs.
KW - Bioanalytics
KW - Bioinformatics
KW - Cyanine dye
KW - DNA
KW - Fluorophore
KW - FRET
KW - Microarray
KW - Photobleaching
KW - ROXS
KW - Scanning
UR - http://www.scopus.com/inward/record.url?scp=85008199898&partnerID=8YFLogxK
U2 - 10.3390/biology5040047
DO - 10.3390/biology5040047
M3 - Article
C2 - 27916881
AN - SCOPUS:85008199898
VL - 5
JO - Biology
JF - Biology
IS - 4
M1 - 47
ER -