Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 13877 |
| Fachzeitschrift | Scientific Reports |
| Jahrgang | 8 |
| Ausgabenummer | 1 |
| Frühes Online-Datum | 17 Sept. 2018 |
| Publikationsstatus | Veröffentlicht - 1 Dez. 2018 |
| Extern publiziert | Ja |
Abstract
Bioprinting is a new technology, which arranges cells with high spatial resolution, but its potential to create models for viral infection studies has not yet been fully realized. The present study describes the optimization of a bioink composition for extrusion printing. The bioinks were biophysically characterized by rheological and electron micrographic measurements. Hydrogels consisting of alginate, gelatin and Matrigel were used to provide a scaffold for a 3D arrangement of human alveolar A549 cells. A blend containing 20% Matrigel provided the optimal conditions for spatial distribution and viability of the printed cells. Infection of the 3D model with a seasonal influenza A strain resulted in widespread distribution of the virus and a clustered infection pattern that is also observed in the natural lung but not in two-dimensional (2D) cell culture, which demonstrates the advantage of 3D printed constructs over conventional culture conditions. The bioink supported viral replication and proinflammatory interferon release of the infected cells. We consider our strategy to be paradigmatic for the generation of humanized 3D tissue models by bioprinting to study infections and develop new antiviral strategies.
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in: Scientific Reports, Jahrgang 8, Nr. 1, 13877, 01.12.2018.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Optimization of cell-laden bioinks for 3D bioprinting and efficient infection with influenza A virus
AU - Berg, Johanna
AU - Hiller, Thomas
AU - Kissner, Maya S.
AU - Qazi, Taimoor H.
AU - Duda, Georg N.
AU - Hocke, Andreas C.
AU - Hippenstiel, Stefan
AU - Elomaa, Laura
AU - Weinhart, Marie
AU - Fahrenson, Christoph
AU - Kurreck, Jens
N1 - Funding Information: Financial support by the “Stiftung zur Förderung der Erforschung von Ersatz-und Ergänzungsmethoden zur Einschränkung von Tierversuchen” (set) and the “Bundesinstitut für Risikoforschung” (1328–568) to J.K. as well as the Einstein center Berlin (kickbox grant to J.B. and T.H.Q.) are gratefully acknowledged. This study is supported by the DFG-SFB-TR84 projects B06 (to A.C.H. and S.H.) and Z01 (to A.C.H.) and by the German Federal Ministry of Education and Research (BMBF) project RAPID (to A.C.H. and S.H.). Furthermore, we thank the “Deutsche Forschungsgemeinschaft” (DFG, INST 131/753-1 FUGG) for financial support of the GeminiSEM 500 microscope. We are particularly thankful to Erik Wade for careful proofreading of the manuscript and helpful comments. We acknowledge support by the German Research Foundation and the Open Access Publication Funds of TU Berlin. Publisher Copyright: © 2018, The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Bioprinting is a new technology, which arranges cells with high spatial resolution, but its potential to create models for viral infection studies has not yet been fully realized. The present study describes the optimization of a bioink composition for extrusion printing. The bioinks were biophysically characterized by rheological and electron micrographic measurements. Hydrogels consisting of alginate, gelatin and Matrigel were used to provide a scaffold for a 3D arrangement of human alveolar A549 cells. A blend containing 20% Matrigel provided the optimal conditions for spatial distribution and viability of the printed cells. Infection of the 3D model with a seasonal influenza A strain resulted in widespread distribution of the virus and a clustered infection pattern that is also observed in the natural lung but not in two-dimensional (2D) cell culture, which demonstrates the advantage of 3D printed constructs over conventional culture conditions. The bioink supported viral replication and proinflammatory interferon release of the infected cells. We consider our strategy to be paradigmatic for the generation of humanized 3D tissue models by bioprinting to study infections and develop new antiviral strategies.
AB - Bioprinting is a new technology, which arranges cells with high spatial resolution, but its potential to create models for viral infection studies has not yet been fully realized. The present study describes the optimization of a bioink composition for extrusion printing. The bioinks were biophysically characterized by rheological and electron micrographic measurements. Hydrogels consisting of alginate, gelatin and Matrigel were used to provide a scaffold for a 3D arrangement of human alveolar A549 cells. A blend containing 20% Matrigel provided the optimal conditions for spatial distribution and viability of the printed cells. Infection of the 3D model with a seasonal influenza A strain resulted in widespread distribution of the virus and a clustered infection pattern that is also observed in the natural lung but not in two-dimensional (2D) cell culture, which demonstrates the advantage of 3D printed constructs over conventional culture conditions. The bioink supported viral replication and proinflammatory interferon release of the infected cells. We consider our strategy to be paradigmatic for the generation of humanized 3D tissue models by bioprinting to study infections and develop new antiviral strategies.
UR - http://www.scopus.com/inward/record.url?scp=85053478828&partnerID=8YFLogxK
U2 - 10.1038/s41598-018-31880-x
DO - 10.1038/s41598-018-31880-x
M3 - Article
C2 - 30224659
AN - SCOPUS:85053478828
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 13877
ER -