Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 101-109 |
| Seitenumfang | 9 |
| Fachzeitschrift | Analytica chimica acta |
| Jahrgang | 163 |
| Ausgabenummer | C |
| Publikationsstatus | Veröffentlicht - 1984 |
| Extern publiziert | Ja |
Abstract
A continuous on-line analysis system for components of the medium during the anaerobic and aerobic fermentation of glucose by Saccharomyces cerevisiae is described. The procedures used include: analysis of the outlet gas by conventional analyzers (CO2, O2) and a mass spectrometer; continuous sampling of the liquid phase with microfiltration and subsequent determination of phosphate and glucose (spectrophotometer), glucose (polarimeter) and ammonium ion (ion-sensitive sensor); and continuous separation of volatile components and dissolved gases with diffusion through a silicone rubber membrane in high vacuum and determination of mass (m/z=18, 28, 31, 32, 40, 44) by means of a mass spectrometer. The system was tested on a batch reactor and a continuously operated three-stage cascade consisting of stirred tank reactors. This equipment was connected to a process computer. Glucose was quantified either polarimetrically or spectrophotometrically with p-hydroxybenzoic acid hydrazide; the latter was far more sensitive and was preferred to enzymatic methods, with which correlation was good. Only acetoin was found to interfere. Problems with operating a mass spectrometer for fermentation control in the exit stream and with membrane sampling are discussed.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Analytische Chemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Umweltwissenschaften (insg.)
- Umweltchemie
- Chemie (insg.)
- Spektroskopie
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in: Analytica chimica acta, Jahrgang 163, Nr. C, 1984, S. 101-109.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - On-line analysis of fermentation media
AU - Schmidt, W. J.
AU - Meyer, H. D.
AU - Schügerl, K.
AU - Kuhlmann, W.
AU - Bellgardt, K. H.
PY - 1984
Y1 - 1984
N2 - A continuous on-line analysis system for components of the medium during the anaerobic and aerobic fermentation of glucose by Saccharomyces cerevisiae is described. The procedures used include: analysis of the outlet gas by conventional analyzers (CO2, O2) and a mass spectrometer; continuous sampling of the liquid phase with microfiltration and subsequent determination of phosphate and glucose (spectrophotometer), glucose (polarimeter) and ammonium ion (ion-sensitive sensor); and continuous separation of volatile components and dissolved gases with diffusion through a silicone rubber membrane in high vacuum and determination of mass (m/z=18, 28, 31, 32, 40, 44) by means of a mass spectrometer. The system was tested on a batch reactor and a continuously operated three-stage cascade consisting of stirred tank reactors. This equipment was connected to a process computer. Glucose was quantified either polarimetrically or spectrophotometrically with p-hydroxybenzoic acid hydrazide; the latter was far more sensitive and was preferred to enzymatic methods, with which correlation was good. Only acetoin was found to interfere. Problems with operating a mass spectrometer for fermentation control in the exit stream and with membrane sampling are discussed.
AB - A continuous on-line analysis system for components of the medium during the anaerobic and aerobic fermentation of glucose by Saccharomyces cerevisiae is described. The procedures used include: analysis of the outlet gas by conventional analyzers (CO2, O2) and a mass spectrometer; continuous sampling of the liquid phase with microfiltration and subsequent determination of phosphate and glucose (spectrophotometer), glucose (polarimeter) and ammonium ion (ion-sensitive sensor); and continuous separation of volatile components and dissolved gases with diffusion through a silicone rubber membrane in high vacuum and determination of mass (m/z=18, 28, 31, 32, 40, 44) by means of a mass spectrometer. The system was tested on a batch reactor and a continuously operated three-stage cascade consisting of stirred tank reactors. This equipment was connected to a process computer. Glucose was quantified either polarimetrically or spectrophotometrically with p-hydroxybenzoic acid hydrazide; the latter was far more sensitive and was preferred to enzymatic methods, with which correlation was good. Only acetoin was found to interfere. Problems with operating a mass spectrometer for fermentation control in the exit stream and with membrane sampling are discussed.
UR - http://www.scopus.com/inward/record.url?scp=0006082339&partnerID=8YFLogxK
U2 - 10.1016/S0003-2670(00)81498-1
DO - 10.1016/S0003-2670(00)81498-1
M3 - Article
AN - SCOPUS:0006082339
VL - 163
SP - 101
EP - 109
JO - Analytica chimica acta
JF - Analytica chimica acta
SN - 0003-2670
IS - C
ER -