TY - JOUR

T1 - Novel magnesium alloy Mg-2La caused no cytotoxic effects on cells in physiological conditions

AU - Weizbauer, Andreas

AU - Seitz, Jan Marten

AU - Werle, Peter

AU - Hegermann, Jan

AU - Willbold, Elmar

AU - Eifler, Rainer

AU - Windhagen, Henning

AU - Reifenrath, Janin

AU - Waizy, Hazibullah

N1 - Funding information: The authors gratefully acknowledge the financial support given by the German Research Foundation (DFG) within the collaborative research project SFB 599 . We thank Dr. Tomas Correa and Dr. Renate Weizbauer for valuable help with language accuracy and Dr. Anneke Loos and Dr. Christina Reufsteck for excellent technical support.

PY - 2014/8/1

Y1 - 2014/8/1

N2 - Using several different in vitro assays, a new biodegradable magnesium alloy Mg-2La, composed of 98% magnesium and 2% lanthanum, was investigated as a possible implant material for biomedical applications. An in vitro cytotoxicity test, according to EN ISO 10993-5/12, with L929 and human osteoblastic cells identified no toxic effects on cell viability at physiological concentrations (at 50% dilutions and higher). The metabolic activity of human osteoblasts in the 100% extract was decreased to < 70% and was therefore rated as cytotoxic. The degradation rates of Mg-2La were evaluated in phosphate buffered saline and four different cell culture media. The degradation rates were shown to be influenced by the composition of the solution, and the addition of fetal bovine serum slightly accelerated the corrosive process. The results of these in vitro experiments suggest that Mg-2La is a promising candidate for use as an orthopedic implant material.

AB - Using several different in vitro assays, a new biodegradable magnesium alloy Mg-2La, composed of 98% magnesium and 2% lanthanum, was investigated as a possible implant material for biomedical applications. An in vitro cytotoxicity test, according to EN ISO 10993-5/12, with L929 and human osteoblastic cells identified no toxic effects on cell viability at physiological concentrations (at 50% dilutions and higher). The metabolic activity of human osteoblasts in the 100% extract was decreased to < 70% and was therefore rated as cytotoxic. The degradation rates of Mg-2La were evaluated in phosphate buffered saline and four different cell culture media. The degradation rates were shown to be influenced by the composition of the solution, and the addition of fetal bovine serum slightly accelerated the corrosive process. The results of these in vitro experiments suggest that Mg-2La is a promising candidate for use as an orthopedic implant material.

KW - Cytotoxicity

KW - Degradation

KW - In vitro

KW - Magnesium alloy

KW - Orthopedic

UR - http://www.scopus.com/inward/record.url?scp=84901064469&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2014.04.063

DO - 10.1016/j.msec.2014.04.063

M3 - Article

C2 - 24907760

AN - SCOPUS:84901064469

VL - 41

SP - 267

EP - 273

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

ER -