TY - JOUR

T1 - Novel chitin scaffolds derived from marine sponge Ianthella basta for tissue engineering approaches based on human mesenchymal stromal cells

T2 - Biocompatibility and cryopreservation

AU - Mutsenko, Vitalii V.

AU - Gryshkov, Oleksandr

AU - Lauterboeck, Lothar

AU - Rogulska, Olena

AU - Tarusin, Dmitriy N.

AU - Bazhenov, Vasilii V.

AU - Schütz, Kathleen

AU - Brüggemeier, Sophie

AU - Gossla, Elke

AU - Akkineni, Ashwini R.

AU - Meißner, Heike

AU - Lode, Anja

AU - Meschke, Stephan

AU - Fromont, Jane

AU - Stelling, Allison L.

AU - Tabachnik, Konstantin R.

AU - Gelinsky, Michael

AU - Nikulin, Sergey

AU - Rodin, Sergey

AU - Tonevitsky, Alexander G.

AU - Petrenko, Alexander Y.

AU - Glasmacher, Birgit

AU - Schupp, Peter J.

AU - Ehrlich, Hermann

N1 - Funding information: The present work was partially supported by Leonhard-Euler-Programm from the German Academic Exchange Service (DAAD, Germany), IP@Leibniz program of the Leibniz Universität Hannover promoted by the DAAD (Germany, project code 57156199) as well as DFG Grant EH 394-3 (DFG, Germany). The authors are very grateful to Igor Kovalenko, Vitaliy Kholodny, Bulat Sydykov, Galina Bozhok and Ortrud Zieschang for the excellent technical assistance.

PY - 2017/11

Y1 - 2017/11

N2 - The extraordinary biocompatibility and mechanical properties of chitinous scaffolds from marine sponges endows these structures with unique properties that render them ideal for diverse biomedical applications. In the present work, a technological route to produce “ready-to-use” tissue-engineered products based on poriferan chitin is comprehensively investigated for the first time. Three key stages included isolation of scaffolds from the marine demosponge Ianthella basta, confirmation of their biocompatibility with human mesenchymal stromal cells, and cryopreservation of the tissue-like structures grown within these scaffolds using a slow cooling protocol. Biocompatibility of the macroporous, flat chitin scaffolds has been confirmed by cell attachment, high cell viability and the ability to differentiate into the adipogenic lineage. The viability of cells cryopreserved on chitin scaffolds was reduced by about 30% as compared to cells cryopreserved in suspension. However, the surviving cells were able to retain their differentiation potential; and this is demonstrated for the adipogenic lineage. The results suggest that chitin from the marine demosponge I. basta is a promising, highly biocompatible biomaterial for stem cell-based tissue-engineering applications.

AB - The extraordinary biocompatibility and mechanical properties of chitinous scaffolds from marine sponges endows these structures with unique properties that render them ideal for diverse biomedical applications. In the present work, a technological route to produce “ready-to-use” tissue-engineered products based on poriferan chitin is comprehensively investigated for the first time. Three key stages included isolation of scaffolds from the marine demosponge Ianthella basta, confirmation of their biocompatibility with human mesenchymal stromal cells, and cryopreservation of the tissue-like structures grown within these scaffolds using a slow cooling protocol. Biocompatibility of the macroporous, flat chitin scaffolds has been confirmed by cell attachment, high cell viability and the ability to differentiate into the adipogenic lineage. The viability of cells cryopreserved on chitin scaffolds was reduced by about 30% as compared to cells cryopreserved in suspension. However, the surviving cells were able to retain their differentiation potential; and this is demonstrated for the adipogenic lineage. The results suggest that chitin from the marine demosponge I. basta is a promising, highly biocompatible biomaterial for stem cell-based tissue-engineering applications.

KW - Chitin

KW - Marine sponges

KW - Mesenchymal stromal cells

KW - Scaffolds

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85018779427&partnerID=8YFLogxK

U2 - 10.1016/j.ijbiomac.2017.03.161

DO - 10.1016/j.ijbiomac.2017.03.161

M3 - Article

C2 - 28365291

AN - SCOPUS:85018779427

VL - 104

SP - 1955

EP - 1965

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -