TY - JOUR

T1 - Novel biodegradable star-shaped polylactide scaffolds for bone regeneration fabricated by two-photon polymerization

AU - Timashev, Peter

AU - Kuznetsova, Daria

AU - Koroleva, Anastasia

AU - Prodanets, Natalia

AU - Deiwick, Andrea

AU - Piskun, Yuri

AU - Bardakova, Ksenia

AU - Dzhoyashvili, Nina

AU - Kostjuk, Sergei

AU - Zagaynova, Elena

AU - Rochev, Yuri

AU - Chichkov, Boris

AU - Bagratashvili, Viktor

N1 - Funding information: This work was supported by the Russian Science Foundation, grant 14-13-01422 (in the part of polymer synthesis, SSL scaffolds forming, SSL scaffolds mechanical analysis, fluorescence analysis of SSL scaffold degradation in vivo grant of the Government of Russian Federation for the Support of Scientific Investigations under the Supervision of Leading Scientists Contract no. 14.B25.31.0019 (in the part of in vitro experiments) and Russian Foundation for Basic Research, grant 15-52-04007 Bel (in the part of in vivo experiments, surgical procedure, histology analysis). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed

PY - 2016/4/14

Y1 - 2016/4/14

N2 - Aim: To assess the properties of 3D biodegradable scaffolds fabricated from novel star-shaped poly(D,L-lactide) (SSL) materials for bone tissue regeneration. Materials & methods: The SSL polymer was synthesized using an optimized synthetic procedure and applied for scaffold fabrication by the two-photon polymerization technique. The osteogenic differentiation was controlled using human adipose-derived stem cells cultured for 28 days. The SSL scaffolds with or without murine MSCs were implanted into the cranial bone of C57/Bl6 mice. Results: The SSL scaffolds supported differentiation of human adipose-derived stem cells toward the osteogenic lineage in vitro. The SSL scaffolds with murine MSCs enhanced the mineralized tissue formation. Conclusion: The SSL scaffolds provide a beneficial microenvironment for the osteogenic MSCs' differentiation in vitro and support de novo bone formation in vivo.

AB - Aim: To assess the properties of 3D biodegradable scaffolds fabricated from novel star-shaped poly(D,L-lactide) (SSL) materials for bone tissue regeneration. Materials & methods: The SSL polymer was synthesized using an optimized synthetic procedure and applied for scaffold fabrication by the two-photon polymerization technique. The osteogenic differentiation was controlled using human adipose-derived stem cells cultured for 28 days. The SSL scaffolds with or without murine MSCs were implanted into the cranial bone of C57/Bl6 mice. Results: The SSL scaffolds supported differentiation of human adipose-derived stem cells toward the osteogenic lineage in vitro. The SSL scaffolds with murine MSCs enhanced the mineralized tissue formation. Conclusion: The SSL scaffolds provide a beneficial microenvironment for the osteogenic MSCs' differentiation in vitro and support de novo bone formation in vivo.

KW - Mscs

KW - osteogenic differentiation

KW - star-shaped polylactide

KW - tissue engineering scaffolds

KW - two-photon polymerization (2PP)

UR - http://www.scopus.com/inward/record.url?scp=84965179546&partnerID=8YFLogxK

U2 - 10.2217/nnm-2015-0022

DO - 10.2217/nnm-2015-0022

M3 - Article

C2 - 27078220

AN - SCOPUS:84965179546

VL - 11

SP - 1041

EP - 1053

JO - Nanomedicine

JF - Nanomedicine

SN - 1743-5889

IS - 9

ER -