Non-invasive in vivo imaging by confocal laser scanning microscopy of gingival tissues following natural plaque deposition

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autorschaft

  • Jörg Eberhard
  • Hendrik Loewen
  • Alexander Krüger
  • Sabine Donner
  • Nico Stumpp
  • Mandy Patzlaff
  • Oliver Stachs
  • Maria Reichard
  • Tammo Ripken
  • Alexander Heisterkamp
  • Meike Stiesch

Organisationseinheiten

Externe Organisationen

  • Medizinische Hochschule Hannover (MHH)
  • Laser Zentrum Hannover e.V. (LZH)
  • Universität Rostock
  • Friedrich-Schiller-Universität Jena
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)321-326
Seitenumfang6
FachzeitschriftJournal of clinical periodontology
Jahrgang41
Ausgabenummer4
PublikationsstatusVeröffentlicht - 7 Jan. 2014

Abstract

Aim Imaging with Confocal Laser Scanning Microscopy (CLSM) generates high-resolution images and may be well suited for basic research in Periodontology and Implant Dentistry. The present study was aimed to explore the in vivo application of CLSM in experimentally induced gingivitis. Materials and Methods Ten subjects were recruited and were advised to stop any oral hygiene of the upper front teeth for 7 days. The gingival tissues were observed using a Heidelberg Retina Tomograph combined with a Rostock Cornea Module at baseline and day 7. The system used a laser of 670 nm and the contrast was given by backscattering from different tissues. Each examination created 800-1200 images that were descriptively analysed. Results After 7 days of abandoned oral hygiene, plaque scores and bleeding frequencies increased. By using CLSM images tooth hard substances, cells and plaque deposits were distinguishable. Increased epithelial cell irregularities, the apical migration of the sulcular epithelium, cellular infiltrates within the sulcus and plaque deposits were observed at day 7. Conclusions The present study showed for the first time that CLSM is suitable for in vivo imaging of the gingival sulcus and adjacent tissues.

ASJC Scopus Sachgebiete

Zitieren

Non-invasive in vivo imaging by confocal laser scanning microscopy of gingival tissues following natural plaque deposition. / Eberhard, Jörg; Loewen, Hendrik; Krüger, Alexander et al.
in: Journal of clinical periodontology, Jahrgang 41, Nr. 4, 07.01.2014, S. 321-326.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Eberhard, J, Loewen, H, Krüger, A, Donner, S, Stumpp, N, Patzlaff, M, Stachs, O, Reichard, M, Ripken, T, Heisterkamp, A & Stiesch, M 2014, 'Non-invasive in vivo imaging by confocal laser scanning microscopy of gingival tissues following natural plaque deposition', Journal of clinical periodontology, Jg. 41, Nr. 4, S. 321-326. https://doi.org/10.1111/jcpe.12227
Eberhard, J., Loewen, H., Krüger, A., Donner, S., Stumpp, N., Patzlaff, M., Stachs, O., Reichard, M., Ripken, T., Heisterkamp, A., & Stiesch, M. (2014). Non-invasive in vivo imaging by confocal laser scanning microscopy of gingival tissues following natural plaque deposition. Journal of clinical periodontology, 41(4), 321-326. https://doi.org/10.1111/jcpe.12227
Eberhard J, Loewen H, Krüger A, Donner S, Stumpp N, Patzlaff M et al. Non-invasive in vivo imaging by confocal laser scanning microscopy of gingival tissues following natural plaque deposition. Journal of clinical periodontology. 2014 Jan 7;41(4):321-326. doi: 10.1111/jcpe.12227
Eberhard, Jörg ; Loewen, Hendrik ; Krüger, Alexander et al. / Non-invasive in vivo imaging by confocal laser scanning microscopy of gingival tissues following natural plaque deposition. in: Journal of clinical periodontology. 2014 ; Jahrgang 41, Nr. 4. S. 321-326.
Download
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AU - Eberhard, Jörg

AU - Loewen, Hendrik

AU - Krüger, Alexander

AU - Donner, Sabine

AU - Stumpp, Nico

AU - Patzlaff, Mandy

AU - Stachs, Oliver

AU - Reichard, Maria

AU - Ripken, Tammo

AU - Heisterkamp, Alexander

AU - Stiesch, Meike

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N2 - Aim Imaging with Confocal Laser Scanning Microscopy (CLSM) generates high-resolution images and may be well suited for basic research in Periodontology and Implant Dentistry. The present study was aimed to explore the in vivo application of CLSM in experimentally induced gingivitis. Materials and Methods Ten subjects were recruited and were advised to stop any oral hygiene of the upper front teeth for 7 days. The gingival tissues were observed using a Heidelberg Retina Tomograph combined with a Rostock Cornea Module at baseline and day 7. The system used a laser of 670 nm and the contrast was given by backscattering from different tissues. Each examination created 800-1200 images that were descriptively analysed. Results After 7 days of abandoned oral hygiene, plaque scores and bleeding frequencies increased. By using CLSM images tooth hard substances, cells and plaque deposits were distinguishable. Increased epithelial cell irregularities, the apical migration of the sulcular epithelium, cellular infiltrates within the sulcus and plaque deposits were observed at day 7. Conclusions The present study showed for the first time that CLSM is suitable for in vivo imaging of the gingival sulcus and adjacent tissues.

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