NMR Methods to Study the Dynamics of SH2 Domain–Phosphopeptide Complexes

Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Beitrag in Buch/Sammelwerk › Forschung › Peer-Review

Autoren

  • Michelangelo Marasco
  • John P. Kirkpatrick
  • Vittoria Nanna
  • Teresa Carlomagno

Organisationseinheiten

Externe Organisationen

  • Memorial Sloan-Kettering Cancer Center
  • University of Birmingham
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Titel des SammelwerksMethods in Molecular Biology
Seiten25-37
Seitenumfang13
PublikationsstatusVeröffentlicht - 2023

Publikationsreihe

NameMethods in Molecular Biology
Band2705
ISSN (Print)1064-3745
ISSN (elektronisch)1940-6029

Abstract

Nuclear magnetic resonance (NMR) spectroscopy is the method of choice for studying the dynamics of biological macromolecules in solution. By exploiting the intricate interplay between the effects of protein motion (both overall rotational diffusion and internal mobility) and nuclear spin relaxation, NMR allows molecular motion to be probed at atomic resolution over a wide range of timescales, including picosecond (bond vibrations and methyl-group rotations), nanosecond (loop motions and rotational diffusion), and microsecond–millisecond (ligand binding, allostery). In this chapter, we describe different NMR pulse schemes (R1, R1ρ, heteronuclear NOE, and CPMG relaxation dispersion) to characterize the dynamics of SH2 domains. As an example, we use the N-SH2 domain of protein tyrosine phosphatase SHP2 in complex with two phosphopeptides derived from immune checkpoint receptor PD-1 (ITIM and ITSM).

ASJC Scopus Sachgebiete

  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Molekularbiologie
  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Genetik

Zitieren

NMR Methods to Study the Dynamics of SH2 Domain–Phosphopeptide Complexes. / Marasco, Michelangelo; Kirkpatrick, John P.; Nanna, Vittoria et al.
Methods in Molecular Biology. 2023. S. 25-37 (Methods in Molecular Biology; Band 2705).

Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Beitrag in Buch/Sammelwerk › Forschung › Peer-Review

Marasco, M, Kirkpatrick, JP, Nanna, V & Carlomagno, T 2023, NMR Methods to Study the Dynamics of SH2 Domain–Phosphopeptide Complexes. in Methods in Molecular Biology. Methods in Molecular Biology, Bd. 2705, S. 25-37. https://doi.org/10.1007/978-1-0716-3393-9_2
Marasco, M., Kirkpatrick, J. P., Nanna, V., & Carlomagno, T. (2023). NMR Methods to Study the Dynamics of SH2 Domain–Phosphopeptide Complexes. In Methods in Molecular Biology (S. 25-37). (Methods in Molecular Biology; Band 2705). https://doi.org/10.1007/978-1-0716-3393-9_2
Marasco M, Kirkpatrick JP, Nanna V, Carlomagno T. NMR Methods to Study the Dynamics of SH2 Domain–Phosphopeptide Complexes. in Methods in Molecular Biology. 2023. S. 25-37. (Methods in Molecular Biology). doi: 10.1007/978-1-0716-3393-9_2
Marasco, Michelangelo ; Kirkpatrick, John P. ; Nanna, Vittoria et al. / NMR Methods to Study the Dynamics of SH2 Domain–Phosphopeptide Complexes. Methods in Molecular Biology. 2023. S. 25-37 (Methods in Molecular Biology).
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AU - Carlomagno, Teresa

N1 - Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023.

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