TY - JOUR

T1 - Nisin application delays growth of Listeria monocytogenes on fresh-cut iceberg lettuce in modified atmosphere packaging, while the bacterial community structure changes within one week of storage

AU - McManamon, Oisin

AU - Kaupper, Thomas

AU - Scollard, Johann

AU - Schmalenberger, Achim

N1 - Funding Information: We would like to thank the Irish Department of Agriculture, Food and the Marine and Safe Food Ireland for financing this study (FIRM 11F008).

PY - 2019/1

Y1 - 2019/1

N2 - Listeria monocytogenes poses a risk to minimally processed ready-to-eat foods such as lettuce due to its ability to grow under refrigeration conditions. Since many natural anti-listerial products render Iceberg lettuce unsuitable for consumption within 2 d of storage, this study investigated the efficacy of Nisin A as anti-listerial agent and its sensory impact on lettuce. In addition, the evolution of the bacterial community on fresh-cut lettuce was monitored for the duration of storage. In-vitro assays confirmed the efficacy of Nisin A to inhibit growth of a three strain mix of L. monocytogenes in model atmospheres and air. The L. monocytogenes strain mix was added to lettuce that was subsequently treated either with Nisin, L. lactis DSM20729 (a Nisin A producer) or was kept without inoculation. Incubation took place at 4 and 8 °C under various atmospheres. On days 0, 2, 5 and 7, L. monocytogenes was enumerated on selective agar and a sensory panel graded the lettuce on visual appearance. At 4 and 8 °C a 10 to 100-fold reduction of L. monocytogenes growth was achieved with 5 mg kg−1 Nisin over a seven-day period, while lettuce kept an acceptable sensory appearance over the first 5 d. Direct application of L. lactis had no detectable effect on L. monocytogenes growth in situ. The bacterial community structure changed substantially from each sampling day to the next over the seven days of incubation. However, Pseudomonadaceae with the genus Pseudomonas were most abundant at all times and increased in relative abundance to over 90% by day 7. In conclusion, the application of Nisin A to minimally processed vegetables like lettuce seems to be a viable alternative to reduce and delay growth of pathogen L. monocytogenes, while not impacting the sensory appearance for 2–5 d.

AB - Listeria monocytogenes poses a risk to minimally processed ready-to-eat foods such as lettuce due to its ability to grow under refrigeration conditions. Since many natural anti-listerial products render Iceberg lettuce unsuitable for consumption within 2 d of storage, this study investigated the efficacy of Nisin A as anti-listerial agent and its sensory impact on lettuce. In addition, the evolution of the bacterial community on fresh-cut lettuce was monitored for the duration of storage. In-vitro assays confirmed the efficacy of Nisin A to inhibit growth of a three strain mix of L. monocytogenes in model atmospheres and air. The L. monocytogenes strain mix was added to lettuce that was subsequently treated either with Nisin, L. lactis DSM20729 (a Nisin A producer) or was kept without inoculation. Incubation took place at 4 and 8 °C under various atmospheres. On days 0, 2, 5 and 7, L. monocytogenes was enumerated on selective agar and a sensory panel graded the lettuce on visual appearance. At 4 and 8 °C a 10 to 100-fold reduction of L. monocytogenes growth was achieved with 5 mg kg−1 Nisin over a seven-day period, while lettuce kept an acceptable sensory appearance over the first 5 d. Direct application of L. lactis had no detectable effect on L. monocytogenes growth in situ. The bacterial community structure changed substantially from each sampling day to the next over the seven days of incubation. However, Pseudomonadaceae with the genus Pseudomonas were most abundant at all times and increased in relative abundance to over 90% by day 7. In conclusion, the application of Nisin A to minimally processed vegetables like lettuce seems to be a viable alternative to reduce and delay growth of pathogen L. monocytogenes, while not impacting the sensory appearance for 2–5 d.

KW - Anti-listerial agent

KW - Lactuca sativa

KW - Modified atmosphere

KW - Next generation sequencing

KW - Nisin A

UR - http://www.scopus.com/inward/record.url?scp=85054564317&partnerID=8YFLogxK

U2 - 10.1016/j.postharvbio.2018.10.002

DO - 10.1016/j.postharvbio.2018.10.002

M3 - Article

AN - SCOPUS:85054564317

VL - 147

SP - 185

EP - 195

JO - Postharvest biology and technology

JF - Postharvest biology and technology

SN - 0925-5214

IS - January 2019

ER -