TY - JOUR

T1 - New 3-D microarray platform based on macroporous polymer monoliths

AU - Rober, M

AU - Walter, J

AU - Vlakh, E

AU - Stahl, F

AU - Kasper, C

AU - Tennikova, T

N1 - Funding information: This work was supported by DFG grant (Grant No. KA 1784/4-1; Coordinators: Prof. T. Tennikova, IMC RAS and Dr. C. Kasper, ITC UH) and RBRF grant (08-08-00876-a, Supervisor Prof. T. Tennikova). The authors are grateful to German Academic Exchange Service (DAAD) for providing the scholarship for PhD student Marina Rober for four-month work at the Institute of Technical Chemistry, University of Hannover (ITC UH). The authors are also grateful to Prof. Thomas Scheper (ITC, Hannover University) and Prof. Valery Krasikov (IMC RAS) for organizing help and fruitful discussions.

PY - 2009/6/30

Y1 - 2009/6/30

N2 - Polymer macroporous monoliths are widely used as efficient sorbents in different, mostly dynamic, interphase processes. In this paper, monolithic materials strongly bound to the inert glass surface are suggested as operative matrices at the development of three-dimensional (3-D) microarrays. For this purpose, several rigid macroporous copolymers differed by reactivity and hydrophobic-hydrophilic properties were synthesized and tested: (1) glycidyl methacrylate-co-ethylene dimethacrylate (poly(GMA-co-EDMA)), (2) glycidyl methacrylate-co-glycerol dimethacrylate (poly(GMA-co-GDMA)), (3) N-hydroxyphthalimide ester of acrylic acid-co-glycidyl methacrylate-co-ethylene dimethacrylate (poly(HPIEAA-co-GMA-co-EDMA)), (4) 2-cyanoethyl methacrylate-co-ethylene dimethacrylate (poly(CEMA-co-EDMA)), and (5) 2-cyanoethyl methacrylate-co-2-hydroxyethyl methacrylate-co-ethylene dimethacrylate (poly(CEMA-co-HEMA-co-EDMA)). The constructed devices were used as platforms for protein microarrays construction and model mouse IgG-goat anti-mouse IgG affinity pair was used to demonstrate the potential of developed test-systems, as well as to optimize microanalytical conditions. The offered microarray platforms were applied to detect the bone tissue marker osteopontin directly in cell culture medium.

AB - Polymer macroporous monoliths are widely used as efficient sorbents in different, mostly dynamic, interphase processes. In this paper, monolithic materials strongly bound to the inert glass surface are suggested as operative matrices at the development of three-dimensional (3-D) microarrays. For this purpose, several rigid macroporous copolymers differed by reactivity and hydrophobic-hydrophilic properties were synthesized and tested: (1) glycidyl methacrylate-co-ethylene dimethacrylate (poly(GMA-co-EDMA)), (2) glycidyl methacrylate-co-glycerol dimethacrylate (poly(GMA-co-GDMA)), (3) N-hydroxyphthalimide ester of acrylic acid-co-glycidyl methacrylate-co-ethylene dimethacrylate (poly(HPIEAA-co-GMA-co-EDMA)), (4) 2-cyanoethyl methacrylate-co-ethylene dimethacrylate (poly(CEMA-co-EDMA)), and (5) 2-cyanoethyl methacrylate-co-2-hydroxyethyl methacrylate-co-ethylene dimethacrylate (poly(CEMA-co-HEMA-co-EDMA)). The constructed devices were used as platforms for protein microarrays construction and model mouse IgG-goat anti-mouse IgG affinity pair was used to demonstrate the potential of developed test-systems, as well as to optimize microanalytical conditions. The offered microarray platforms were applied to detect the bone tissue marker osteopontin directly in cell culture medium.

KW - Animals

KW - Goats

KW - Immunoglobulin G/chemistry

KW - Mice

KW - Osteopontin/analysis

KW - Polymers/chemical synthesis

KW - Protein Array Analysis/methods

U2 - 10.1016/j.aca.2009.04.015

DO - 10.1016/j.aca.2009.04.015

M3 - Article

C2 - 19463569

VL - 644

SP - 95

EP - 103

JO - Analytica chimica acta

JF - Analytica chimica acta

SN - 0003-2670

IS - 1-2

ER -