Details
| Originalsprache | Englisch |
|---|---|
| Titel des Sammelwerks | Optical Interactions with Tissue and Cells XVI |
| Untertitel | 22 - 26 January 2005, San Jose, California, USA |
| Erscheinungsort | Bellingham |
| Herausgeber (Verlag) | SPIE |
| Seiten | 230-235 |
| Seitenumfang | 6 |
| ISBN (Print) | 0-8194-5669-1 |
| Publikationsstatus | Veröffentlicht - 15 Apr. 2005 |
| Extern publiziert | Ja |
| Veranstaltung | Optical Interactions with Tissue and Cells XVI - San Jose, CA, USA / Vereinigte Staaten Dauer: 24 Jan. 2005 → 26 Jan. 2005 |
Publikationsreihe
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Herausgeber (Verlag) | SPIE |
| Band | 5695 |
| ISSN (Print) | 1605-7422 |
Abstract
We selectively disrupted the cytoskeletal network of fixed and live bovine capillary endothelial cell using ultrashort laser pulses. We image the microtubules in the cytoskeleton of the cultured cells using green fluorescent protein. The cells are placed on a custom-built inverted fluorescence microscope setup, using a 1.4 NA oil-immersion objective to both image the cell and focus the laser radiation into the cell samples. The laser delivers 100-fs laser pulses centered at 800 nm at a repetition rate of 1 kHz; the typical energy delivered at the sample is 1-5nJ. The fluorescent image of the cell is captured with a CCD-camera at one frame per second. To determine the spatial discrimination of the laser cutting we ablated microtubules and actin fibers in fixed cells. At pulse energies below 2 nJ we obtain an ablation size of 200 nm. This low pulse energy and high spatial discrimination enable the application of this technique to live cells. We severed a single microtubule inside the live cells without affecting the cell's viability. The targeted microtubule snaps and depolymerizes after the cutting. This nanosurgery technique will further the understanding and modeling of stress and compression in the cytoskeletal network of live cells.
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Elektronische, optische und magnetische Materialien
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Physik und Astronomie (insg.)
- Atom- und Molekularphysik sowie Optik
- Medizin (insg.)
- Radiologie, Nuklearmedizin und Bildgebung
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Optical Interactions with Tissue and Cells XVI: 22 - 26 January 2005, San Jose, California, USA. Bellingham: SPIE, 2005. S. 230-235 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Band 5695).
Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Aufsatz in Konferenzband › Forschung › Peer-Review
}
TY - GEN
T1 - Nanosurgery in live cells using ultrashort laser pulses
AU - Heisterkamp, A.
AU - Maxwell, I. Z.
AU - Kumar, Sanjay
AU - Underwood, Jean M.
AU - Nickerson, Jeffrey A.
AU - Ingber, D. E.
AU - Mazur, E.
PY - 2005/4/15
Y1 - 2005/4/15
N2 - We selectively disrupted the cytoskeletal network of fixed and live bovine capillary endothelial cell using ultrashort laser pulses. We image the microtubules in the cytoskeleton of the cultured cells using green fluorescent protein. The cells are placed on a custom-built inverted fluorescence microscope setup, using a 1.4 NA oil-immersion objective to both image the cell and focus the laser radiation into the cell samples. The laser delivers 100-fs laser pulses centered at 800 nm at a repetition rate of 1 kHz; the typical energy delivered at the sample is 1-5nJ. The fluorescent image of the cell is captured with a CCD-camera at one frame per second. To determine the spatial discrimination of the laser cutting we ablated microtubules and actin fibers in fixed cells. At pulse energies below 2 nJ we obtain an ablation size of 200 nm. This low pulse energy and high spatial discrimination enable the application of this technique to live cells. We severed a single microtubule inside the live cells without affecting the cell's viability. The targeted microtubule snaps and depolymerizes after the cutting. This nanosurgery technique will further the understanding and modeling of stress and compression in the cytoskeletal network of live cells.
AB - We selectively disrupted the cytoskeletal network of fixed and live bovine capillary endothelial cell using ultrashort laser pulses. We image the microtubules in the cytoskeleton of the cultured cells using green fluorescent protein. The cells are placed on a custom-built inverted fluorescence microscope setup, using a 1.4 NA oil-immersion objective to both image the cell and focus the laser radiation into the cell samples. The laser delivers 100-fs laser pulses centered at 800 nm at a repetition rate of 1 kHz; the typical energy delivered at the sample is 1-5nJ. The fluorescent image of the cell is captured with a CCD-camera at one frame per second. To determine the spatial discrimination of the laser cutting we ablated microtubules and actin fibers in fixed cells. At pulse energies below 2 nJ we obtain an ablation size of 200 nm. This low pulse energy and high spatial discrimination enable the application of this technique to live cells. We severed a single microtubule inside the live cells without affecting the cell's viability. The targeted microtubule snaps and depolymerizes after the cutting. This nanosurgery technique will further the understanding and modeling of stress and compression in the cytoskeletal network of live cells.
KW - Ablation
KW - Actin
KW - Biology
KW - Cell surgery
KW - Microtubule
KW - Photodisruption
KW - Ultrashort laser pulses
UR - http://www.scopus.com/inward/record.url?scp=23244441634&partnerID=8YFLogxK
U2 - 10.1117/12.590467
DO - 10.1117/12.590467
M3 - Conference contribution
AN - SCOPUS:23244441634
SN - 0-8194-5669-1
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
SP - 230
EP - 235
BT - Optical Interactions with Tissue and Cells XVI
PB - SPIE
CY - Bellingham
T2 - Optical Interactions with Tissue and Cells XVI
Y2 - 24 January 2005 through 26 January 2005
ER -