Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | e202000037 |
| Fachzeitschrift | Journal of Biophotonics |
| Jahrgang | 13 |
| Ausgabenummer | 7 |
| Frühes Online-Datum | 6 Apr. 2020 |
| Publikationsstatus | Veröffentlicht - 2 Juli 2020 |
Abstract
Controlling cell adhesion and cell differentiation is necessary to fabricate a tissue with arbitrary properties for tissue engineering applications. A substrate with a porous structure as a cell scaffold allows the diffusion of the cell culture medium through the scaffold. In this work, we show that the femtosecond laser fabricated micro through-holes in biodegradable polymer films, enhance myoblast adhesion, and accelerates proliferation and differentiation. ChR2-C2C12 and UT-C2C12 cells were seeded on the films with micro through-holes each fabricated by a single femtosecond laser pulse. Cell adhesion was enhanced on films with holes fabricated by laser irradiation. In addition, cell proliferation was accelerated on films with micro through-holes that penetrate the film, compared to on films with micro craters that do not penetrate the film. On films with arrays consisting of micro through-holes, cells aligned along the arrays and cell fusion was enhanced, indicating the acceleration of cell differentiation.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Allgemeine Chemie
- Werkstoffwissenschaften (insg.)
- Allgemeine Materialwissenschaften
- Biochemie, Genetik und Molekularbiologie (insg.)
- Allgemeine Biochemie, Genetik und Molekularbiologie
- Ingenieurwesen (insg.)
- Allgemeiner Maschinenbau
- Physik und Astronomie (insg.)
- Allgemeine Physik und Astronomie
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in: Journal of Biophotonics, Jahrgang 13, Nr. 7, e202000037, 02.07.2020.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Myoblast adhesion and proliferation on biodegradable polymer films with femtosecond laser-fabricated micro through-holes
AU - Takayama, Izumi
AU - Kondo, Naonari
AU - Kalies, Stefan
AU - Heisterkamp, Alexander
AU - Terakawa, Mitsuhiro
N1 - Funding Information: This research was funded in part by KAKENHI (No. 18H03551).
PY - 2020/7/2
Y1 - 2020/7/2
N2 - Controlling cell adhesion and cell differentiation is necessary to fabricate a tissue with arbitrary properties for tissue engineering applications. A substrate with a porous structure as a cell scaffold allows the diffusion of the cell culture medium through the scaffold. In this work, we show that the femtosecond laser fabricated micro through-holes in biodegradable polymer films, enhance myoblast adhesion, and accelerates proliferation and differentiation. ChR2-C2C12 and UT-C2C12 cells were seeded on the films with micro through-holes each fabricated by a single femtosecond laser pulse. Cell adhesion was enhanced on films with holes fabricated by laser irradiation. In addition, cell proliferation was accelerated on films with micro through-holes that penetrate the film, compared to on films with micro craters that do not penetrate the film. On films with arrays consisting of micro through-holes, cells aligned along the arrays and cell fusion was enhanced, indicating the acceleration of cell differentiation.
AB - Controlling cell adhesion and cell differentiation is necessary to fabricate a tissue with arbitrary properties for tissue engineering applications. A substrate with a porous structure as a cell scaffold allows the diffusion of the cell culture medium through the scaffold. In this work, we show that the femtosecond laser fabricated micro through-holes in biodegradable polymer films, enhance myoblast adhesion, and accelerates proliferation and differentiation. ChR2-C2C12 and UT-C2C12 cells were seeded on the films with micro through-holes each fabricated by a single femtosecond laser pulse. Cell adhesion was enhanced on films with holes fabricated by laser irradiation. In addition, cell proliferation was accelerated on films with micro through-holes that penetrate the film, compared to on films with micro craters that do not penetrate the film. On films with arrays consisting of micro through-holes, cells aligned along the arrays and cell fusion was enhanced, indicating the acceleration of cell differentiation.
KW - C2C12 cells
KW - cell adhesion
KW - cell differentiation
KW - cell proliferation
KW - femtosecond laser ablation
KW - micro through-holes
UR - http://www.scopus.com/inward/record.url?scp=85083419406&partnerID=8YFLogxK
U2 - 10.1002/jbio.202000037
DO - 10.1002/jbio.202000037
M3 - Article
AN - SCOPUS:85083419406
VL - 13
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 7
M1 - e202000037
ER -