TY - JOUR

T1 - Mycoremediation affects antioxidative status in winter rye plants grown at Chernobyl exclusion zone site in Ukraine

AU - Vuković, Ana

AU - Schulz, Wolfgang

AU - Čamagajevac, Ivna Štolfa

AU - Gaur, Apoorva

AU - Walther, Clemens

AU - Gupta, Dharmendra K.

N1 - Funding Information: The authors greatly acknowledge Dr. Johannes Raff of Institute for Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany, for providing two different macro fungi for the experiments. Authors also acknowledge Dr. Sergiy Dubchak, State Specialized Enterprise “Radon,” Kyiv, Ukraine, and Dr. Igor Chyzhevsky, State Specialized Enterprise “Eco-center,” Chornobyl, Ukraine, for their help during this experiment and in access of Chornobyl exclusion zone.

PY - 2020/7

Y1 - 2020/7

N2 - Soil contaminated with heavy metals in general and radionuclides in particular represents an escalating problem for all living organisms. Since, Chernobyl nuclear power plant accident in 1986 in Ukraine, an exclusion zone of 30 km around the former power plant is uninhabitable land due to severe contamination. Two most notable beta emitters contributing to dose hazards for decades is radioactive 137Cs/90Sr. However, large parts of the zone are also highly contaminated with uranium particles (hot particles) bearing trace amounts of highly alpha-emitting radionuclides. We established an experiment at exclusion zone with the aim to investigate the influence of two macro fungi (Schizophyllum commune (S.C.) and Leucoagaricus naucinus (L.N.)) on oxidative status and antioxidative responses in winter rye plants; from this, we wanted to test the radionuclide/heavy metals retention capacity of both fungi, and probe their further potential for mycoremediation. Result shows some differences in the concentrations of radionuclides/heavy metals and micro/macronutrients uptake in plants. As a biomarker of oxidative status, lipid peroxidation (LPO) levels and other antioxidative parameters were determined, i.e., superoxide-dismutase (SOD) isoenzymes, cysteine (CYS), and ascorbic acid (AA) concentrations as well as catalase (CAT) and glutathione reductase (GR) activities in winter rye shoots. LPO showed no significant differences between controls and plants cultivated with macro fungi. However, CAT activities were elevated in the presence of S.C/L.N compared with control, while GR activity was significantly higher only in presence of S.C. In contrast, isozyme of SOD (Cu,Zn-SOD) was the most prominent in control. Likewise, CYS content was lower in plants grown with both fungi, while AA concentration was only lower in the presence of L.N. The results showed that presence of fungi in radionuclide contaminated soil caused induction of antioxidative response in shoots of winter rye and that the response depended on the type of fungi used.

AB - Soil contaminated with heavy metals in general and radionuclides in particular represents an escalating problem for all living organisms. Since, Chernobyl nuclear power plant accident in 1986 in Ukraine, an exclusion zone of 30 km around the former power plant is uninhabitable land due to severe contamination. Two most notable beta emitters contributing to dose hazards for decades is radioactive 137Cs/90Sr. However, large parts of the zone are also highly contaminated with uranium particles (hot particles) bearing trace amounts of highly alpha-emitting radionuclides. We established an experiment at exclusion zone with the aim to investigate the influence of two macro fungi (Schizophyllum commune (S.C.) and Leucoagaricus naucinus (L.N.)) on oxidative status and antioxidative responses in winter rye plants; from this, we wanted to test the radionuclide/heavy metals retention capacity of both fungi, and probe their further potential for mycoremediation. Result shows some differences in the concentrations of radionuclides/heavy metals and micro/macronutrients uptake in plants. As a biomarker of oxidative status, lipid peroxidation (LPO) levels and other antioxidative parameters were determined, i.e., superoxide-dismutase (SOD) isoenzymes, cysteine (CYS), and ascorbic acid (AA) concentrations as well as catalase (CAT) and glutathione reductase (GR) activities in winter rye shoots. LPO showed no significant differences between controls and plants cultivated with macro fungi. However, CAT activities were elevated in the presence of S.C/L.N compared with control, while GR activity was significantly higher only in presence of S.C. In contrast, isozyme of SOD (Cu,Zn-SOD) was the most prominent in control. Likewise, CYS content was lower in plants grown with both fungi, while AA concentration was only lower in the presence of L.N. The results showed that presence of fungi in radionuclide contaminated soil caused induction of antioxidative response in shoots of winter rye and that the response depended on the type of fungi used.

KW - Chernobyl exclusion zone

KW - Leucoagaricus naucinus

KW - Mycoremediation

KW - Oxidative stress

KW - Schizophyllum commune

KW - Winter rye plant

UR - http://www.scopus.com/inward/record.url?scp=85084647841&partnerID=8YFLogxK

U2 - 10.1007/s11356-020-09137-w

DO - 10.1007/s11356-020-09137-w

M3 - Article

C2 - 32399885

AN - SCOPUS:85084647841

VL - 27

SP - 25818

EP - 25827

JO - Environmental Science and Pollution Research

JF - Environmental Science and Pollution Research

SN - 0944-1344

IS - 20

ER -