TY - GEN

T1 - Multiplex PCR as useful tool for characterization of interspecific hybrids of Petunia and Calibrachoa

AU - Jȩdrzejuk, A.

AU - Meyer, Lina

AU - Serek, Margrethe

N1 - Funding information: The experimental work was partly supported by the DAAD grant no. KPZ A/08/73505. The authors would like to thank Prof. em. Bjarne M. Stummann (University of Copenhagen, Denmark) for his valuable advice and critical review of the manuscript. The authors would also like to thank the technical and scientific staff from Institute of Horticulture Production Systems, Section of Floriculture at Leibniz University Hannover for technical assistance and valuable advice concerning manuscript preparation. The experimental work was partly supported by the DAAD grant no. KPZ A/ ? ?/ ? ? ? ? ?. The authors would like to thank Prof. em. Bjarne M. Stummann (University of Copenhagen, Denmark) for his valuable advice and critical review of the manuscript. The authors would also like to thank the technical and scientific staff from Institute of Horticulture Production Systems, Section of Floriculture at Leibniz University Hannover for technical assistance and valuable advice concerning manuscript preparation.

PY - 2018/4/30

Y1 - 2018/4/30

N2 - Petunia and Calibrachoa are ornamental plants of international economic importance. Commercially, it is advantageous to combine desirable features of Calibrachoa, such as diversity of flower colors, with the pH tolerance and vigorous growth of Petunia. Currently on the floricultural market several commercial hybrids between Petunia and Calibrachoa exist, such as 'Supercal' or 'Calitunia'. However, even though 'Supercal' and 'Calitunia' are recognized as hybrids of Petunia and Calibrachoa, it is not generally known how they were obtained. A variety of different methods is available for hybrid identification between closely related genera. To detect closely related genera, exon sequences are too similar or even identical, whereas intron sequences may differ sufficiently in length and sequence to be used in the amplification of PCR fragments that can distinguish between sequences derived from the parent species. In this study we clearly demonstrate that PCR amplicons from selected introns with flanking exon regions can, in fact, be used to identify and study hybrids of Petunia and Calibrachoa. Four different introns with flanking exon areas were used, each from a different gene and with a different sequence for Petunia and Calibrachoa, as genus specific markers for chromosomes of interspecific hybrids. PCR primers specific for Petunia and Calabrachoa were constructed from determined sequences and tested on a range of presumed hybrids using multiplex PCR, which confirmed that the hybrids were derived from these two species. Similar technology can be used for the molecular characterization of other, new hybrids formed between closely related species or cultivars.

AB - Petunia and Calibrachoa are ornamental plants of international economic importance. Commercially, it is advantageous to combine desirable features of Calibrachoa, such as diversity of flower colors, with the pH tolerance and vigorous growth of Petunia. Currently on the floricultural market several commercial hybrids between Petunia and Calibrachoa exist, such as 'Supercal' or 'Calitunia'. However, even though 'Supercal' and 'Calitunia' are recognized as hybrids of Petunia and Calibrachoa, it is not generally known how they were obtained. A variety of different methods is available for hybrid identification between closely related genera. To detect closely related genera, exon sequences are too similar or even identical, whereas intron sequences may differ sufficiently in length and sequence to be used in the amplification of PCR fragments that can distinguish between sequences derived from the parent species. In this study we clearly demonstrate that PCR amplicons from selected introns with flanking exon regions can, in fact, be used to identify and study hybrids of Petunia and Calibrachoa. Four different introns with flanking exon areas were used, each from a different gene and with a different sequence for Petunia and Calibrachoa, as genus specific markers for chromosomes of interspecific hybrids. PCR primers specific for Petunia and Calabrachoa were constructed from determined sequences and tested on a range of presumed hybrids using multiplex PCR, which confirmed that the hybrids were derived from these two species. Similar technology can be used for the molecular characterization of other, new hybrids formed between closely related species or cultivars.

KW - Hybrid identification

KW - Intron-exon area

KW - Solanaceae

UR - http://www.scopus.com/inward/record.url?scp=85048808051&partnerID=8YFLogxK

U2 - 10.17660/ActaHortic.2018.1201.79

DO - 10.17660/ActaHortic.2018.1201.79

M3 - Conference contribution

AN - SCOPUS:85048808051

T3 - Acta Horticulturae

SP - 591

EP - 597

BT - 7th International Conference on Managing Quality in Chains (MQUIC2017) and 2nd International Symposium on Ornamentals in association with 13th International Protea Research Symposium

A2 - Reinten, E.

A2 - Tsige, A.A.

A2 - Fawole, O.A.

A2 - Hoffman, E.W.

A2 - Opara, U.L.

A2 - de Kock, L.

PB - International Society for Horticultural Science

T2 - VII International Conference on Managing Quality in Chains (MQUIC2017) and II International Symposium on Ornamentals in association with XIII International Protea Research Symposium

Y2 - 4 September 2017 through 7 September 2017

ER -