TY - JOUR

T1 - Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy

AU - Fedorov Kukk, Anatoly

AU - Wu, Di

AU - Gaffal, Evelyn

AU - Panzer, Rüdiger

AU - Emmert, Steffen

AU - Roth, Bernhard

N1 - Funding Information: Deutsche Forschungsgemeinschaft, Grant/Award Numbers: RO 3471/18‐1, EM 63/13‐1; German Research Foundation (DFG) under Germany's Excellence Strategy within the Cluster of Excellence PhoenixD, Grant/Award Number: EXC 2122, Project ID 390833453 Funding information Funding Information: The authors acknowledge financial support from the German Research Foundation DFG (German Research Foundation, Project ID RO 3471/18‐1 and EM 63/13‐1). Also, financial support from the German Research Foundation (DFG) under Germany's Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453) is acknowledged.

PY - 2022/10/3

Y1 - 2022/10/3

N2 - We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement. The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma. The RS and OCT utilize the same scanning and optical setup, allowing for co-localized measurements. The US on the other side is integrated with an acoustical reflector, which enables B-mode measurements on the same position as OCT and RS. The US B-mode scans can be translated across the sample by laterally moving the US transducer, which is made possible by the developed adapter with a flexible membrane. We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.

AB - We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement. The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma. The RS and OCT utilize the same scanning and optical setup, allowing for co-localized measurements. The US on the other side is integrated with an acoustical reflector, which enables B-mode measurements on the same position as OCT and RS. The US B-mode scans can be translated across the sample by laterally moving the US transducer, which is made possible by the developed adapter with a flexible membrane. We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.

KW - multimodal setup

KW - optical coherence tomography

KW - Raman spectroscopy

KW - skin cancer diagnostics

KW - ultrasound

UR - http://www.scopus.com/inward/record.url?scp=85135020920&partnerID=8YFLogxK

U2 - 10.1002/jbio.202200129

DO - 10.1002/jbio.202200129

M3 - Article

C2 - 35802400

AN - SCOPUS:85135020920

VL - 15

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 10

M1 - e202200129

ER -