Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1801-1808 |
| Seitenumfang | 8 |
| Fachzeitschrift | The journal of immunology |
| Jahrgang | 197 |
| Ausgabenummer | 5 |
| Publikationsstatus | Veröffentlicht - 1 Sept. 2016 |
Abstract
The mucosal immune system is relevant for homeostasis, immunity, and also pathological conditions in the gastrointestinal tract. Inducible NO synthase (iNOS)-dependent production of NO is one of the factors linked to both antimicrobial immunity and pathological conditions. Upregulation of iNOS has been observed in human Helicobacter pylori infection, but the cellular sources of iNOS are ill defined. Key differences in regulation of iNOS expression impair the translation from mouse models to human medicine. To characterize mucosal iNOS-producing leukocytes, biopsy specimens from H. pylori-infected patients, controls, and participants of a vaccination trial were analyzed by immunohistochemistry, along with flow cytometric analyses of lymphocytes for iNOS expression and activity. We newly identified mucosal IgA-producing plasma cells (PCs) as one major iNOS + cell population in H. pylori-infected patients and confirmed intracellular NO production. Because we did not detect iNOS + PCs in three distinct infectious diseases, this is not a general feature of mucosal PCs under conditions of infection. Furthermore, numbers of mucosal iNOS + PCs were elevated in individuals who had cleared experimental H. pylori infection compared with those who had not. Thus, IgA + PCs expressing iNOS are described for the first time, to our knowledge, in humans. iNOS + PCs are induced in the course of human H. pylori infection, and their abundance seems to correlate with the clinical course of the infection.
ASJC Scopus Sachgebiete
- Medizin (insg.)
- Immunologie und Allergologie
- Immunologie und Mikrobiologie (insg.)
- Immunologie
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in: The journal of immunology, Jahrgang 197, Nr. 5, 01.09.2016, S. 1801-1808.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Mucosal inducible NO synthase–producing iga+ plasma cells in helicobacter pylori–infected patients
AU - Neumann, Laura
AU - Mueller, M.
AU - Moos, V.
AU - Heller, Frank
AU - Meyer, Thomas F.
AU - Loddenkemper, C.
AU - Bojarski, C.
AU - Fehlings, M.
AU - Doerner, T.
AU - Allers, K.
AU - Aebischer, Toni
AU - Ignatius, R.
AU - Schneider, T.
N1 - Publisher Copyright: Copyright © 2016 by The American Association of Immunologists, Inc. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016/9/1
Y1 - 2016/9/1
N2 - The mucosal immune system is relevant for homeostasis, immunity, and also pathological conditions in the gastrointestinal tract. Inducible NO synthase (iNOS)-dependent production of NO is one of the factors linked to both antimicrobial immunity and pathological conditions. Upregulation of iNOS has been observed in human Helicobacter pylori infection, but the cellular sources of iNOS are ill defined. Key differences in regulation of iNOS expression impair the translation from mouse models to human medicine. To characterize mucosal iNOS-producing leukocytes, biopsy specimens from H. pylori-infected patients, controls, and participants of a vaccination trial were analyzed by immunohistochemistry, along with flow cytometric analyses of lymphocytes for iNOS expression and activity. We newly identified mucosal IgA-producing plasma cells (PCs) as one major iNOS + cell population in H. pylori-infected patients and confirmed intracellular NO production. Because we did not detect iNOS + PCs in three distinct infectious diseases, this is not a general feature of mucosal PCs under conditions of infection. Furthermore, numbers of mucosal iNOS + PCs were elevated in individuals who had cleared experimental H. pylori infection compared with those who had not. Thus, IgA + PCs expressing iNOS are described for the first time, to our knowledge, in humans. iNOS + PCs are induced in the course of human H. pylori infection, and their abundance seems to correlate with the clinical course of the infection.
AB - The mucosal immune system is relevant for homeostasis, immunity, and also pathological conditions in the gastrointestinal tract. Inducible NO synthase (iNOS)-dependent production of NO is one of the factors linked to both antimicrobial immunity and pathological conditions. Upregulation of iNOS has been observed in human Helicobacter pylori infection, but the cellular sources of iNOS are ill defined. Key differences in regulation of iNOS expression impair the translation from mouse models to human medicine. To characterize mucosal iNOS-producing leukocytes, biopsy specimens from H. pylori-infected patients, controls, and participants of a vaccination trial were analyzed by immunohistochemistry, along with flow cytometric analyses of lymphocytes for iNOS expression and activity. We newly identified mucosal IgA-producing plasma cells (PCs) as one major iNOS + cell population in H. pylori-infected patients and confirmed intracellular NO production. Because we did not detect iNOS + PCs in three distinct infectious diseases, this is not a general feature of mucosal PCs under conditions of infection. Furthermore, numbers of mucosal iNOS + PCs were elevated in individuals who had cleared experimental H. pylori infection compared with those who had not. Thus, IgA + PCs expressing iNOS are described for the first time, to our knowledge, in humans. iNOS + PCs are induced in the course of human H. pylori infection, and their abundance seems to correlate with the clinical course of the infection.
UR - http://www.scopus.com/inward/record.url?scp=84983784390&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.1501330
DO - 10.4049/jimmunol.1501330
M3 - Article
VL - 197
SP - 1801
EP - 1808
JO - The journal of immunology
JF - The journal of immunology
SN - 0022-1767
IS - 5
ER -