Monitoring of NADH-dependent culture fluorescence during the cultivation of Escherichia coli

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

Organisationseinheiten

Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)B7-B12
FachzeitschriftThe Chemical Engineering Journal
Jahrgang34
Ausgabenummer1
PublikationsstatusVeröffentlicht - Jan. 1987

Abstract

NADH-dependent culture fluorescence was monitored during various E. coli cultivation processes (batch and continuous cultivation in stirred tank reactors or bubble column reactors). Various strains of E. coli (including genetically modified E. coli) were used. Measurement of the fluorescence signal allows biomass estimation under certain conditions. The monitoring of culture fluorescence is useful for obtaining information on the metabolic status of the cells. The effect on the cells of different substrates (glucose and oxygen) was studied. The measurements were performed in synthetic and complex media and the influence of the composition of each medium was investigated. A microfluorometer was used as a routine sensor for cultivation control and provided insight into the status of the cells.

ASJC Scopus Sachgebiete

Zitieren

Monitoring of NADH-dependent culture fluorescence during the cultivation of Escherichia coli. / Scheper, Th; Gebauer, A.; Schügerl, K.
in: The Chemical Engineering Journal, Jahrgang 34, Nr. 1, 01.1987, S. B7-B12.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Download
@article{65e2eddae5c448bf90893bec9ca4ce4d,
title = "Monitoring of NADH-dependent culture fluorescence during the cultivation of Escherichia coli",
abstract = "NADH-dependent culture fluorescence was monitored during various E. coli cultivation processes (batch and continuous cultivation in stirred tank reactors or bubble column reactors). Various strains of E. coli (including genetically modified E. coli) were used. Measurement of the fluorescence signal allows biomass estimation under certain conditions. The monitoring of culture fluorescence is useful for obtaining information on the metabolic status of the cells. The effect on the cells of different substrates (glucose and oxygen) was studied. The measurements were performed in synthetic and complex media and the influence of the composition of each medium was investigated. A microfluorometer was used as a routine sensor for cultivation control and provided insight into the status of the cells.",
author = "Th Scheper and A. Gebauer and K. Sch{\"u}gerl",
year = "1987",
month = jan,
doi = "10.1016/0300-9467(87)85009-8",
language = "English",
volume = "34",
pages = "B7--B12",
journal = "The Chemical Engineering Journal",
issn = "0300-9467",
publisher = "Elsevier",
number = "1",

}

Download

TY - JOUR

T1 - Monitoring of NADH-dependent culture fluorescence during the cultivation of Escherichia coli

AU - Scheper, Th

AU - Gebauer, A.

AU - Schügerl, K.

PY - 1987/1

Y1 - 1987/1

N2 - NADH-dependent culture fluorescence was monitored during various E. coli cultivation processes (batch and continuous cultivation in stirred tank reactors or bubble column reactors). Various strains of E. coli (including genetically modified E. coli) were used. Measurement of the fluorescence signal allows biomass estimation under certain conditions. The monitoring of culture fluorescence is useful for obtaining information on the metabolic status of the cells. The effect on the cells of different substrates (glucose and oxygen) was studied. The measurements were performed in synthetic and complex media and the influence of the composition of each medium was investigated. A microfluorometer was used as a routine sensor for cultivation control and provided insight into the status of the cells.

AB - NADH-dependent culture fluorescence was monitored during various E. coli cultivation processes (batch and continuous cultivation in stirred tank reactors or bubble column reactors). Various strains of E. coli (including genetically modified E. coli) were used. Measurement of the fluorescence signal allows biomass estimation under certain conditions. The monitoring of culture fluorescence is useful for obtaining information on the metabolic status of the cells. The effect on the cells of different substrates (glucose and oxygen) was studied. The measurements were performed in synthetic and complex media and the influence of the composition of each medium was investigated. A microfluorometer was used as a routine sensor for cultivation control and provided insight into the status of the cells.

UR - http://www.scopus.com/inward/record.url?scp=0023108617&partnerID=8YFLogxK

U2 - 10.1016/0300-9467(87)85009-8

DO - 10.1016/0300-9467(87)85009-8

M3 - Article

AN - SCOPUS:0023108617

VL - 34

SP - B7-B12

JO - The Chemical Engineering Journal

JF - The Chemical Engineering Journal

SN - 0300-9467

IS - 1

ER -

Von denselben Autoren