TY - JOUR

T1 - Mitomycin C-induced effects on aerobic methanotrophs in a landfill cover soil

T2 - implications of a viral shunt?

AU - Heffner, Tanja

AU - Kaupper, Thomas

AU - Heinrichs, Mara

AU - Lee, Hyo Jung

AU - Rüppel, Nadine

AU - Horn, Marcus A

AU - Ho, Adrian

N1 - Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft (HO6234/1-2), and the Leibniz Universität Hannover, Germany.

PY - 2023/6

Y1 - 2023/6

N2 - A viral shunt can occur when phages going through a lytic cycle, including lysogenic phages triggered by inducing agents (e.g. mitomycin C), results in host lysis and the release of cell constituents and virions. The impact of a viral shunt on the carbon, including methane cycle in soil systems is poorly understood. Here, we determined the effects of mitomycin C on the aerobic methanotrophs in a landfill cover soil. To an extent, our results support a mitomycin C-induced viral shunt, as indicated by the significantly higher viral-like particle (VLP) counts relative to bacteria, elevated nutrient concentrations (ammonium, succinate), and initially impaired microbial activities (methane uptake and microbial respiration) after mitomycin C addition. The trend in microbial activities at <2 days largely corresponded to the expression of the pmoA and 16S rRNA genes. Thereafter (>11 days), the active bacterial community composition significantly diverged in the mitomycin C-supplemented incubations, suggesting the differential impact of mitomycin C on the bacterial community. Collectively, we provide insight on the effects of mitomycin C, and potentially a viral shunt, on the bacteria in the soil environment.

AB - A viral shunt can occur when phages going through a lytic cycle, including lysogenic phages triggered by inducing agents (e.g. mitomycin C), results in host lysis and the release of cell constituents and virions. The impact of a viral shunt on the carbon, including methane cycle in soil systems is poorly understood. Here, we determined the effects of mitomycin C on the aerobic methanotrophs in a landfill cover soil. To an extent, our results support a mitomycin C-induced viral shunt, as indicated by the significantly higher viral-like particle (VLP) counts relative to bacteria, elevated nutrient concentrations (ammonium, succinate), and initially impaired microbial activities (methane uptake and microbial respiration) after mitomycin C addition. The trend in microbial activities at <2 days largely corresponded to the expression of the pmoA and 16S rRNA genes. Thereafter (>11 days), the active bacterial community composition significantly diverged in the mitomycin C-supplemented incubations, suggesting the differential impact of mitomycin C on the bacterial community. Collectively, we provide insight on the effects of mitomycin C, and potentially a viral shunt, on the bacteria in the soil environment.

KW - landfill cover

KW - methane

KW - methylobacter

KW - methylocystis

KW - methylosarcina

KW - pmoA

KW - viral shunt

UR - http://www.scopus.com/inward/record.url?scp=85163087409&partnerID=8YFLogxK

U2 - 10.1093/femsec/fiad047

DO - 10.1093/femsec/fiad047

M3 - Article

C2 - 37156510

VL - 99

JO - FEMS microbiology ecology

JF - FEMS microbiology ecology

SN - 0168-6496

IS - 6

M1 - fiad047

ER -