Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant

Publikation: Beitrag in Buch/Bericht/Sammelwerk/KonferenzbandBeitrag in Buch/SammelwerkForschungPeer-Review

Autoren

Organisationseinheiten

Externe Organisationen

  • Karlsruher Institut für Technologie (KIT)
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Titel des SammelwerksStem Cell Mobilization
UntertitelMethods and Protocols
Herausgeber (Verlag)Humana Press
Seiten97-107
Seitenumfang11
Auflage1
ISBN (elektronisch)978-1-4939-9574-5
ISBN (Print)978-1-4939-9573-8, 978-1-4939-9576-9
PublikationsstatusVeröffentlicht - 14 Juni 2019

Publikationsreihe

NameMethods in Molecular Biology
Band2017
ISSN (Print)1064-3745
ISSN (elektronisch)1940-6029

Abstract

In leukemia, leukemic cells hijack the hematopoietic stem cell (HSC) microenvironment in the bone marrow—the so-called stem cell niche—by flooding the niche with clonal progeny of leukemic cells. They can exploit signaling pathways which are critical for HSC development to support their own survival, homing, and maintenance. These interactions of leukemic cells with the microenvironment have an impact on therapy progress and patient outcome. Therefore, signals for homing and anchorage of leukemic cells to the bone marrow have to be investigated by using tools that allow the migration of cells toward critical signals. Here, we describe an in vitro migration assay for leukemic cells toward a chemoattractant in a 3D environment exemplified by migration of the cell line OCI-AML3 to a CXC motif chemokine ligand 12 (CXCL12) gradient. For this purpose, a chemotaxis slide is filled with a hydrogel system mimicking the extracellular matrix in vivo. The cells are encapsulated into the hydrogel network during polymerization, and a CXCL12 gradient is introduced in the enclosed chambers to trigger migration. Cell migration in the 3D network of the hydrogel is monitored by time-lapse microscopy. We describe the experimental setup and the tools for cell tracking and data analysis.

ASJC Scopus Sachgebiete

  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Genetik
  • Biochemie, Genetik und Molekularbiologie (insg.)
  • Molekularbiologie

Zitieren

Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant. / Zippel, Sabrina; Raic, Annamarija; Lee-Thedieck, Cornelia.
Stem Cell Mobilization: Methods and Protocols. 1. Aufl. Humana Press, 2019. S. 97-107 (Methods in Molecular Biology; Band 2017).

Publikation: Beitrag in Buch/Bericht/Sammelwerk/KonferenzbandBeitrag in Buch/SammelwerkForschungPeer-Review

Zippel, S, Raic, A & Lee-Thedieck, C 2019, Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant. in Stem Cell Mobilization: Methods and Protocols. 1 Aufl., Methods in Molecular Biology, Bd. 2017, Humana Press, S. 97-107. https://doi.org/10.1007/978-1-4939-9574-5_8
Zippel, S., Raic, A., & Lee-Thedieck, C. (2019). Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant. In Stem Cell Mobilization: Methods and Protocols (1 Aufl., S. 97-107). (Methods in Molecular Biology; Band 2017). Humana Press. https://doi.org/10.1007/978-1-4939-9574-5_8
Zippel S, Raic A, Lee-Thedieck C. Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant. in Stem Cell Mobilization: Methods and Protocols. 1 Aufl. Humana Press. 2019. S. 97-107. (Methods in Molecular Biology). doi: 10.1007/978-1-4939-9574-5_8
Zippel, Sabrina ; Raic, Annamarija ; Lee-Thedieck, Cornelia. / Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant. Stem Cell Mobilization: Methods and Protocols. 1. Aufl. Humana Press, 2019. S. 97-107 (Methods in Molecular Biology).
Download
@inbook{7f8bb2e9fec24eb2ba106d022f5df419,
title = "Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant",
abstract = "In leukemia, leukemic cells hijack the hematopoietic stem cell (HSC) microenvironment in the bone marrow—the so-called stem cell niche—by flooding the niche with clonal progeny of leukemic cells. They can exploit signaling pathways which are critical for HSC development to support their own survival, homing, and maintenance. These interactions of leukemic cells with the microenvironment have an impact on therapy progress and patient outcome. Therefore, signals for homing and anchorage of leukemic cells to the bone marrow have to be investigated by using tools that allow the migration of cells toward critical signals. Here, we describe an in vitro migration assay for leukemic cells toward a chemoattractant in a 3D environment exemplified by migration of the cell line OCI-AML3 to a CXC motif chemokine ligand 12 (CXCL12) gradient. For this purpose, a chemotaxis slide is filled with a hydrogel system mimicking the extracellular matrix in vivo. The cells are encapsulated into the hydrogel network during polymerization, and a CXCL12 gradient is introduced in the enclosed chambers to trigger migration. Cell migration in the 3D network of the hydrogel is monitored by time-lapse microscopy. We describe the experimental setup and the tools for cell tracking and data analysis.",
keywords = "Leukemic cells, Migration, 3D matrix, mu-Slides, CXCR4/CXCL12 axis, Chemokine gradient, μ-Slides",
author = "Sabrina Zippel and Annamarija Raic and Cornelia Lee-Thedieck",
year = "2019",
month = jun,
day = "14",
doi = "10.1007/978-1-4939-9574-5_8",
language = "English",
isbn = "978-1-4939-9573-8",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "97--107",
booktitle = "Stem Cell Mobilization",
address = "United States",
edition = "1",

}

Download

TY - CHAP

T1 - Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant

AU - Zippel, Sabrina

AU - Raic, Annamarija

AU - Lee-Thedieck, Cornelia

PY - 2019/6/14

Y1 - 2019/6/14

N2 - In leukemia, leukemic cells hijack the hematopoietic stem cell (HSC) microenvironment in the bone marrow—the so-called stem cell niche—by flooding the niche with clonal progeny of leukemic cells. They can exploit signaling pathways which are critical for HSC development to support their own survival, homing, and maintenance. These interactions of leukemic cells with the microenvironment have an impact on therapy progress and patient outcome. Therefore, signals for homing and anchorage of leukemic cells to the bone marrow have to be investigated by using tools that allow the migration of cells toward critical signals. Here, we describe an in vitro migration assay for leukemic cells toward a chemoattractant in a 3D environment exemplified by migration of the cell line OCI-AML3 to a CXC motif chemokine ligand 12 (CXCL12) gradient. For this purpose, a chemotaxis slide is filled with a hydrogel system mimicking the extracellular matrix in vivo. The cells are encapsulated into the hydrogel network during polymerization, and a CXCL12 gradient is introduced in the enclosed chambers to trigger migration. Cell migration in the 3D network of the hydrogel is monitored by time-lapse microscopy. We describe the experimental setup and the tools for cell tracking and data analysis.

AB - In leukemia, leukemic cells hijack the hematopoietic stem cell (HSC) microenvironment in the bone marrow—the so-called stem cell niche—by flooding the niche with clonal progeny of leukemic cells. They can exploit signaling pathways which are critical for HSC development to support their own survival, homing, and maintenance. These interactions of leukemic cells with the microenvironment have an impact on therapy progress and patient outcome. Therefore, signals for homing and anchorage of leukemic cells to the bone marrow have to be investigated by using tools that allow the migration of cells toward critical signals. Here, we describe an in vitro migration assay for leukemic cells toward a chemoattractant in a 3D environment exemplified by migration of the cell line OCI-AML3 to a CXC motif chemokine ligand 12 (CXCL12) gradient. For this purpose, a chemotaxis slide is filled with a hydrogel system mimicking the extracellular matrix in vivo. The cells are encapsulated into the hydrogel network during polymerization, and a CXCL12 gradient is introduced in the enclosed chambers to trigger migration. Cell migration in the 3D network of the hydrogel is monitored by time-lapse microscopy. We describe the experimental setup and the tools for cell tracking and data analysis.

KW - Leukemic cells

KW - Migration

KW - 3D matrix

KW - mu-Slides

KW - CXCR4/CXCL12 axis

KW - Chemokine gradient

KW - μ-Slides

UR - http://www.scopus.com/inward/record.url?scp=85067521033&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9574-5_8

DO - 10.1007/978-1-4939-9574-5_8

M3 - Contribution to book/anthology

SN - 978-1-4939-9573-8

SN - 978-1-4939-9576-9

T3 - Methods in Molecular Biology

SP - 97

EP - 107

BT - Stem Cell Mobilization

PB - Humana Press

ER -

Von denselben Autoren