TY - JOUR

T1 - Microbial stoichiometric flexibility regulates rice straw mineralization and its priming effect in paddy soil

AU - Zhu, Zhenke

AU - Ge, Tida

AU - Luo, Yu

AU - Liu, Shoulong

AU - Xu, Xingliang

AU - Tong, Chengli

AU - Shibistova, Olga

AU - Guggenberger, Georg

AU - Wu, Jinshui

N1 - Funding information: This study was supported by the National Key Research and Development program (grant number: 2016YFE0101100 ); the National Natural Science Foundation of China (grant numbers: 41430860 , 41501321 , 41371304 ); the Youth Innovation Team Project of the Institute of Subtropical Agriculture , Chinese Academy of Sciences (grant number: 2017QNCXTD_GTD ); and Chinese Academy of Sciences President's International Fellowship Initiative to Georg Guggenberger ( 2018VCA0031 ). We thank the Public Service Technology Center, the Institute of Subtropical Agriculture, and the Chinese Academy of Sciences for technical assistance.

PY - 2018/6

Y1 - 2018/6

N2 - Nitrogen (N) and phosphorus (P) availability plays a crucial role in carbon (C) cycling in terrestrial ecosystems. However, the C:N:P stoichiometric regulation of microbial mineralization of plant residues and its impact on the soil priming effect (PE), measured as CO2 and CH4 emission, in paddy soils remain unclear. In this study, the effect of soil C:N:P stoichiometry (regulated by the application of N and P fertilizers) on the mineralization of 13C-labelled rice straw and the subsequent PE was investigated in a 100-day incubation experiment in flooded paddy soil. N and P additions increased straw mineralization by approximately 25% and 10%, respectively. Additions of both N and P led to higher CO2 efflux, but lower CH4 emission. With an increase in the ratios of DOC:NH4+-N, DOC:Olsen P, and microbial biomass C:N, 13CO2 efflux increased exponentially to a maximum. Compared with sole straw addition, exclusive N addition led to a weaker PE for CO2 emission, whereas exclusive P addition induced a stronger PE for CO2 emission. In contrast, CH4 emitted from native soil organic matter (SOM) was reduced by 7.4% and 46.1% following P and NP application, respectively. Structural equation models suggest that available N had dominant and direct positive effects, whereas microbial biomass stoichiometry mainly exerted negative indirect effects on PE. The stoichiometry of soil enzyme activity directly down-regulated CH4 emission from SOM. Microbes obviously regulate soil C turnover via stoichiometric flexibility to maintain an elemental stoichiometric balance between resources and microbial requirements. The addition of straw in combination with N and P fertilization in paddy soils could therefore meet microbial stoichiometric requirements and regulate microbial activity and extracellular enzyme production, resulting in co-metabolism of fresh C and native SOM.

AB - Nitrogen (N) and phosphorus (P) availability plays a crucial role in carbon (C) cycling in terrestrial ecosystems. However, the C:N:P stoichiometric regulation of microbial mineralization of plant residues and its impact on the soil priming effect (PE), measured as CO2 and CH4 emission, in paddy soils remain unclear. In this study, the effect of soil C:N:P stoichiometry (regulated by the application of N and P fertilizers) on the mineralization of 13C-labelled rice straw and the subsequent PE was investigated in a 100-day incubation experiment in flooded paddy soil. N and P additions increased straw mineralization by approximately 25% and 10%, respectively. Additions of both N and P led to higher CO2 efflux, but lower CH4 emission. With an increase in the ratios of DOC:NH4+-N, DOC:Olsen P, and microbial biomass C:N, 13CO2 efflux increased exponentially to a maximum. Compared with sole straw addition, exclusive N addition led to a weaker PE for CO2 emission, whereas exclusive P addition induced a stronger PE for CO2 emission. In contrast, CH4 emitted from native soil organic matter (SOM) was reduced by 7.4% and 46.1% following P and NP application, respectively. Structural equation models suggest that available N had dominant and direct positive effects, whereas microbial biomass stoichiometry mainly exerted negative indirect effects on PE. The stoichiometry of soil enzyme activity directly down-regulated CH4 emission from SOM. Microbes obviously regulate soil C turnover via stoichiometric flexibility to maintain an elemental stoichiometric balance between resources and microbial requirements. The addition of straw in combination with N and P fertilization in paddy soils could therefore meet microbial stoichiometric requirements and regulate microbial activity and extracellular enzyme production, resulting in co-metabolism of fresh C and native SOM.

KW - Element stoichiometry

KW - Extracellular enzyme activity

KW - Priming effect

KW - Soil microbial biomass

KW - Soil organic matter turnover

KW - Structural equation models

UR - http://www.scopus.com/inward/record.url?scp=85043303375&partnerID=8YFLogxK

U2 - 10.1016/j.soilbio.2018.03.003

DO - 10.1016/j.soilbio.2018.03.003

M3 - Article

AN - SCOPUS:85043303375

VL - 121

SP - 67

EP - 76

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

ER -