TY - JOUR

T1 - Microarray-based expression analysis of phytohormone-related genes in rice seedlings during cyanide metabolism

AU - Yu, Xiao Zhang

AU - Lin, Yu Juan

AU - Lu, Chun Jiao

AU - Gupta, Dharmendra K.

N1 - Funding information: This work was financially supported by the National Natural Science Foundation of China (No. 41761094) and The Guangxi Talent Highland for Hazardous Waste Disposal Industrialization.

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Plants exhibit highly coordinated, dynamic reactions to various abiotic stressors. As cyanide is a non-essential element for plant growth, entry inside plants can exert toxicity at multiple levels. In plant, hormone plays a pivot role under stress conditions. The fluctuations of stress-responsive hormones help in altering cellular dynamics and hence play a central role in coordination and adaptation growth responses under stress. This study focusses on uptake of cyanide in Oryza sativa seedlings and its effect on physiological and on genetic level. Microarray approach has been focused on transcriptional profiling of genes which are involved in systemic acquired resistance for cyanide. Our study shows that the change in different hormonal contents maintained almost the same pattern in roots and shoots upon CN exposure, except for SA. However, the hormone-related gene expression pattern conducted by microarray analysis was inconsistent in both plant materials (root/shoots). Comparison of gene expression between root/shoots showed a total of 29 in roots and 16 DEGs, respectively, indicating that hormone-related genes in roots were more responsive than those in shoots during exogenous CN metabolism. These results showed a remarkable change at transcript level of plant hormone-related genes, including biosynthesis, degradation, induction, and signal transduction under cyanide stress.

AB - Plants exhibit highly coordinated, dynamic reactions to various abiotic stressors. As cyanide is a non-essential element for plant growth, entry inside plants can exert toxicity at multiple levels. In plant, hormone plays a pivot role under stress conditions. The fluctuations of stress-responsive hormones help in altering cellular dynamics and hence play a central role in coordination and adaptation growth responses under stress. This study focusses on uptake of cyanide in Oryza sativa seedlings and its effect on physiological and on genetic level. Microarray approach has been focused on transcriptional profiling of genes which are involved in systemic acquired resistance for cyanide. Our study shows that the change in different hormonal contents maintained almost the same pattern in roots and shoots upon CN exposure, except for SA. However, the hormone-related gene expression pattern conducted by microarray analysis was inconsistent in both plant materials (root/shoots). Comparison of gene expression between root/shoots showed a total of 29 in roots and 16 DEGs, respectively, indicating that hormone-related genes in roots were more responsive than those in shoots during exogenous CN metabolism. These results showed a remarkable change at transcript level of plant hormone-related genes, including biosynthesis, degradation, induction, and signal transduction under cyanide stress.

KW - Abiotic stress

KW - Cyanide

KW - O. sativa

KW - Phytohormone

KW - Signal transduction

UR - http://www.scopus.com/inward/record.url?scp=85046553367&partnerID=8YFLogxK

U2 - 10.1007/s11356-018-2118-8

DO - 10.1007/s11356-018-2118-8

M3 - Article

C2 - 29736647

AN - SCOPUS:85046553367

VL - 25

SP - 19701

EP - 19712

JO - Environmental Science and Pollution Research

JF - Environmental Science and Pollution Research

SN - 0944-1344

IS - 20

ER -