Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autoren

  • Julia Santiago
  • Benjamin Brandt
  • Mari Wildhagen
  • Ulrich Hohmann
  • Ludwig A. Hothorn
  • Melinka A. Butenko
  • Michael Hothorn

Organisationseinheiten

Externe Organisationen

  • Universität Genf
  • University of Oslo
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummere15075
FachzeitschriftELIFE
Jahrgang5
AusgabenummerApril 2016
PublikationsstatusVeröffentlicht - 8 Apr. 2016

Abstract

Plants constantly renew during their life cycle and thus require to shed senescent and damaged organs. Floral abscission is controlled by the leucine-rich repeat receptor kinase (LRR-RK) HAESA and the peptide hormone IDA. It is unknown how expression of IDA in the abscission zone leads to HAESA activation. Here we show that IDA is sensed directly by the HAESA ectodomain. Crystal structures of HAESA in complex with IDA reveal a hormone binding pocket that accommodates an active dodecamer peptide. A central hydroxyproline residue anchors IDA to the receptor. The HAESA co-receptor SERK1, a positive regulator of the floral abscission pathway, allows for high-affinity sensing of the peptide hormone by binding to an Arg-His-Asn motif in IDA. This sequence pattern is conserved among diverse plant peptides, suggesting that plant peptide hormone receptors may share a common ligand binding mode and activation mechanism.

ASJC Scopus Sachgebiete

Zitieren

Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission. / Santiago, Julia; Brandt, Benjamin; Wildhagen, Mari et al.
in: ELIFE, Jahrgang 5, Nr. April 2016, e15075, 08.04.2016.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Santiago, J, Brandt, B, Wildhagen, M, Hohmann, U, Hothorn, LA, Butenko, MA & Hothorn, M 2016, 'Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission', ELIFE, Jg. 5, Nr. April 2016, e15075. https://doi.org/10.7554/eLife.15075, https://doi.org/10.15488/619
Santiago, J., Brandt, B., Wildhagen, M., Hohmann, U., Hothorn, L. A., Butenko, M. A., & Hothorn, M. (2016). Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission. ELIFE, 5(April 2016), Artikel e15075. https://doi.org/10.7554/eLife.15075, https://doi.org/10.15488/619
Santiago J, Brandt B, Wildhagen M, Hohmann U, Hothorn LA, Butenko MA et al. Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission. ELIFE. 2016 Apr 8;5(April 2016):e15075. doi: 10.7554/eLife.15075, 10.15488/619
Santiago, Julia ; Brandt, Benjamin ; Wildhagen, Mari et al. / Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission. in: ELIFE. 2016 ; Jahrgang 5, Nr. April 2016.
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abstract = "Plants constantly renew during their life cycle and thus require to shed senescent and damaged organs. Floral abscission is controlled by the leucine-rich repeat receptor kinase (LRR-RK) HAESA and the peptide hormone IDA. It is unknown how expression of IDA in the abscission zone leads to HAESA activation. Here we show that IDA is sensed directly by the HAESA ectodomain. Crystal structures of HAESA in complex with IDA reveal a hormone binding pocket that accommodates an active dodecamer peptide. A central hydroxyproline residue anchors IDA to the receptor. The HAESA co-receptor SERK1, a positive regulator of the floral abscission pathway, allows for high-affinity sensing of the peptide hormone by binding to an Arg-His-Asn motif in IDA. This sequence pattern is conserved among diverse plant peptides, suggesting that plant peptide hormone receptors may share a common ligand binding mode and activation mechanism.",
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