Details
| Originalsprache | Englisch |
|---|---|
| Titel des Sammelwerks | Frontiers in Ultrafast Optics |
| Untertitel | Biomedical, Scientific, and Industrial Applications XI |
| Publikationsstatus | Veröffentlicht - 11 Feb. 2011 |
| Veranstaltung | Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI - San Francisco, CA, USA / Vereinigte Staaten Dauer: 23 Jan. 2011 → 26 Jan. 2011 |
Publikationsreihe
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Band | 7925 |
| ISSN (Print) | 0277-786X |
Abstract
The gold nanoparticle (AuNP) mediated ultrashort laser cell membrane perforation has been proven as an efficient delivery method to bring membrane impermeable molecules into the cytoplasm. Nevertheless, the underlying mechanisms have not been fully determined yet. Different effects may occur when irradiating a AuNP with ultrashort laser pulses and finally enable the molecule to transfer. Depending on the parameters (pulse length, laser fluence and wavelength, particle size and shape, etc.) light absorption or an enhanced near field scattering can lead to perforation of the cell membrane when the particle is in close vicinity. Here we present our experimental results to clarify the perforation initiating mechanisms. The generation of cavitation and gas bubbles due to the laser induced effects were observed via time resolved imaging. Additionally, pump-probe experiments for bubble detection was performed. Furthermore, in our patch clamp studies a depolarization of the membrane potential and the current through the membrane of AuNP loaded cell during laser treatment was detected. This indicates an exchange of extra- and intra cellular ions trough the perforated cell membrane for some milliseconds. Additionally investigations by ESEM imaging were applied to study the interaction of cells and AuNP after co incubation. The images show an attachment of AuNP at the cell membrane after several hours of incubation. Moreover, images of irradiated and AuNP loaded cells were taken to visualize the laser induced effects.
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Elektronische, optische und magnetische Materialien
- Physik und Astronomie (insg.)
- Physik der kondensierten Materie
- Informatik (insg.)
- Angewandte Informatik
- Mathematik (insg.)
- Angewandte Mathematik
- Ingenieurwesen (insg.)
- Elektrotechnik und Elektronik
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Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI. 2011. 79250F (Proceedings of SPIE - The International Society for Optical Engineering; Band 7925).
Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Aufsatz in Konferenzband › Forschung › Peer-Review
}
TY - GEN
T1 - Mechanisms of gold nanoparticle mediated ultrashort laser cell membrane perforation
AU - Schomaker, M.
AU - Baumgart, J.
AU - Motekaitis, D.
AU - Heinemann, D.
AU - Krawinkel, J.
AU - Pangalos, Maria
AU - Bintig, Willem
AU - Boulais, E.
AU - Lachaine, R.
AU - St.-Louis Lalonde, B.
AU - Ngezahayo, Anaclet
AU - Meunier, M.
AU - Heisterkamp, Alexander
PY - 2011/2/11
Y1 - 2011/2/11
N2 - The gold nanoparticle (AuNP) mediated ultrashort laser cell membrane perforation has been proven as an efficient delivery method to bring membrane impermeable molecules into the cytoplasm. Nevertheless, the underlying mechanisms have not been fully determined yet. Different effects may occur when irradiating a AuNP with ultrashort laser pulses and finally enable the molecule to transfer. Depending on the parameters (pulse length, laser fluence and wavelength, particle size and shape, etc.) light absorption or an enhanced near field scattering can lead to perforation of the cell membrane when the particle is in close vicinity. Here we present our experimental results to clarify the perforation initiating mechanisms. The generation of cavitation and gas bubbles due to the laser induced effects were observed via time resolved imaging. Additionally, pump-probe experiments for bubble detection was performed. Furthermore, in our patch clamp studies a depolarization of the membrane potential and the current through the membrane of AuNP loaded cell during laser treatment was detected. This indicates an exchange of extra- and intra cellular ions trough the perforated cell membrane for some milliseconds. Additionally investigations by ESEM imaging were applied to study the interaction of cells and AuNP after co incubation. The images show an attachment of AuNP at the cell membrane after several hours of incubation. Moreover, images of irradiated and AuNP loaded cells were taken to visualize the laser induced effects.
AB - The gold nanoparticle (AuNP) mediated ultrashort laser cell membrane perforation has been proven as an efficient delivery method to bring membrane impermeable molecules into the cytoplasm. Nevertheless, the underlying mechanisms have not been fully determined yet. Different effects may occur when irradiating a AuNP with ultrashort laser pulses and finally enable the molecule to transfer. Depending on the parameters (pulse length, laser fluence and wavelength, particle size and shape, etc.) light absorption or an enhanced near field scattering can lead to perforation of the cell membrane when the particle is in close vicinity. Here we present our experimental results to clarify the perforation initiating mechanisms. The generation of cavitation and gas bubbles due to the laser induced effects were observed via time resolved imaging. Additionally, pump-probe experiments for bubble detection was performed. Furthermore, in our patch clamp studies a depolarization of the membrane potential and the current through the membrane of AuNP loaded cell during laser treatment was detected. This indicates an exchange of extra- and intra cellular ions trough the perforated cell membrane for some milliseconds. Additionally investigations by ESEM imaging were applied to study the interaction of cells and AuNP after co incubation. The images show an attachment of AuNP at the cell membrane after several hours of incubation. Moreover, images of irradiated and AuNP loaded cells were taken to visualize the laser induced effects.
KW - biophotonics
KW - cavitation bubbles
KW - cell manipulation
KW - ESEM imaging
KW - nanoparticles
KW - patch clamp
KW - perforation
KW - perforation mechanisms
KW - plasmonics
KW - transfection
KW - ultrashort laser pulses
UR - http://www.scopus.com/inward/record.url?scp=79953868850&partnerID=8YFLogxK
U2 - 10.1117/12.876625
DO - 10.1117/12.876625
M3 - Conference contribution
AN - SCOPUS:79953868850
SN - 9780819484628
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Frontiers in Ultrafast Optics
T2 - Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XI
Y2 - 23 January 2011 through 26 January 2011
ER -