Details
| Originalsprache | Englisch |
|---|---|
| Titel des Sammelwerks | Optical Interactions with Tissue and Cells XXII |
| Publikationsstatus | Veröffentlicht - 8 Feb. 2011 |
| Extern publiziert | Ja |
| Veranstaltung | Optical Interactions with Tissue and Cells XXII - San Francisco, CA, USA / Vereinigte Staaten Dauer: 24 Jan. 2011 → 26 Jan. 2011 |
Publikationsreihe
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Band | 7897 |
| ISSN (Print) | 1605-7422 |
Abstract
Although femtosecond laser cell surgery is widely used for fundamental research in cell biology, the mechanisms in the so-called low-density plasma regime are largely unknown. To date, it is still unclear on which time scales free electron and free radical-induced chemical effects take place leading to intracellular ablation. In this paper, we present our experimental study on the influence of laser parameters and staining on the ablation threshold. We found that the ablation effect resulted from the accumulation of single-shot multiphoton-induced photochemical effects finished within a few nanoseconds. In addition, fluorescence staining of subcellular structures significantly decreased the ablation threshold. Based on our findings, we propose that dye molecules are the major source for providing seed electrons for the ionization cascade.
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Elektronische, optische und magnetische Materialien
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Physik und Astronomie (insg.)
- Atom- und Molekularphysik sowie Optik
- Medizin (insg.)
- Radiologie, Nuklearmedizin und Bildgebung
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- BibTex
- RIS
Optical Interactions with Tissue and Cells XXII. 2011. 789704 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Band 7897).
Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Aufsatz in Konferenzband › Forschung › Peer-Review
}
TY - GEN
T1 - Mechanisms of femtosecond laser cell surgery in the low-density plasma regime
AU - Kuetemeyer, Kai
AU - Rezgui, R.
AU - Lubatschowski, Holger
AU - Heisterkamp, Alexander
PY - 2011/2/8
Y1 - 2011/2/8
N2 - Although femtosecond laser cell surgery is widely used for fundamental research in cell biology, the mechanisms in the so-called low-density plasma regime are largely unknown. To date, it is still unclear on which time scales free electron and free radical-induced chemical effects take place leading to intracellular ablation. In this paper, we present our experimental study on the influence of laser parameters and staining on the ablation threshold. We found that the ablation effect resulted from the accumulation of single-shot multiphoton-induced photochemical effects finished within a few nanoseconds. In addition, fluorescence staining of subcellular structures significantly decreased the ablation threshold. Based on our findings, we propose that dye molecules are the major source for providing seed electrons for the ionization cascade.
AB - Although femtosecond laser cell surgery is widely used for fundamental research in cell biology, the mechanisms in the so-called low-density plasma regime are largely unknown. To date, it is still unclear on which time scales free electron and free radical-induced chemical effects take place leading to intracellular ablation. In this paper, we present our experimental study on the influence of laser parameters and staining on the ablation threshold. We found that the ablation effect resulted from the accumulation of single-shot multiphoton-induced photochemical effects finished within a few nanoseconds. In addition, fluorescence staining of subcellular structures significantly decreased the ablation threshold. Based on our findings, we propose that dye molecules are the major source for providing seed electrons for the ionization cascade.
KW - Cell surgery
KW - Femtosecond laser
KW - Free radicals
KW - Low-density plasma
KW - Photochemistry
UR - http://www.scopus.com/inward/record.url?scp=79955143224&partnerID=8YFLogxK
U2 - 10.1117/12.874147
DO - 10.1117/12.874147
M3 - Conference contribution
AN - SCOPUS:79955143224
SN - 9780819484345
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optical Interactions with Tissue and Cells XXII
T2 - Optical Interactions with Tissue and Cells XXII
Y2 - 24 January 2011 through 26 January 2011
ER -