Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 155-164 |
| Seitenumfang | 10 |
| Fachzeitschrift | Molecular breeding |
| Jahrgang | 4 |
| Ausgabenummer | 2 |
| Publikationsstatus | Veröffentlicht - Apr. 1998 |
Abstract
We examined the resistance phenotype of a large number of transgenic tobacco plants originating from 12 commercial (Nicotiana tabacum) cultivars expressing the sense form of the nucleoprotein (N) gene of L3, a Bulgarian isolate of tomato spotted wilt virus (TSWV). The analysis revealed that transgenic plants are completely protected against the homologous L3 isolate of TSWV irrespective of whether or not they contain detectable levels of translational product. The effectiveness of protection against the virus was investigated upon mechanical inoculation under greenhouse conditions and in field trials. Non-segregating resistant lines were selected and the inheritance of the resistance to TSWV was analysed in successive generations (R3-R6). Extensive tests under controlled conditions and two-year field trials proved that the resistance to TSWV is stable in different environments and is a stably inherited trait.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Molekularbiologie
- Agrar- und Biowissenschaften (insg.)
- Agronomie und Nutzpflanzenwissenschaften
- Biochemie, Genetik und Molekularbiologie (insg.)
- Genetik
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
in: Molecular breeding, Jahrgang 4, Nr. 2, 04.1998, S. 155-164.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Long-term resistance to tomato spotted wilt virus in transgenic tobacco cultivars expressing the viral nucleoprotein gene
T2 - Greenhouse and field tests
AU - Stoeva, Pravda
AU - Yankulova, Margarita
AU - Nikolaeva, Velichka
AU - Bachvarova, Rositza
AU - Ivanova, Lidia
AU - Maiss, Edgar
AU - Adam, Guenter
AU - Vulkov, Vladimir
AU - Guelemerov, Spas
AU - Atanassov, Atanas
PY - 1998/4
Y1 - 1998/4
N2 - We examined the resistance phenotype of a large number of transgenic tobacco plants originating from 12 commercial (Nicotiana tabacum) cultivars expressing the sense form of the nucleoprotein (N) gene of L3, a Bulgarian isolate of tomato spotted wilt virus (TSWV). The analysis revealed that transgenic plants are completely protected against the homologous L3 isolate of TSWV irrespective of whether or not they contain detectable levels of translational product. The effectiveness of protection against the virus was investigated upon mechanical inoculation under greenhouse conditions and in field trials. Non-segregating resistant lines were selected and the inheritance of the resistance to TSWV was analysed in successive generations (R3-R6). Extensive tests under controlled conditions and two-year field trials proved that the resistance to TSWV is stable in different environments and is a stably inherited trait.
AB - We examined the resistance phenotype of a large number of transgenic tobacco plants originating from 12 commercial (Nicotiana tabacum) cultivars expressing the sense form of the nucleoprotein (N) gene of L3, a Bulgarian isolate of tomato spotted wilt virus (TSWV). The analysis revealed that transgenic plants are completely protected against the homologous L3 isolate of TSWV irrespective of whether or not they contain detectable levels of translational product. The effectiveness of protection against the virus was investigated upon mechanical inoculation under greenhouse conditions and in field trials. Non-segregating resistant lines were selected and the inheritance of the resistance to TSWV was analysed in successive generations (R3-R6). Extensive tests under controlled conditions and two-year field trials proved that the resistance to TSWV is stable in different environments and is a stably inherited trait.
KW - Field trials
KW - Nucleoprotein gene-mediated resistance
KW - Transgenic tobacco
KW - TSWV
UR - http://www.scopus.com/inward/record.url?scp=7144227295&partnerID=8YFLogxK
U2 - 10.1023/A:1009644315295
DO - 10.1023/A:1009644315295
M3 - Article
AN - SCOPUS:7144227295
VL - 4
SP - 155
EP - 164
JO - Molecular breeding
JF - Molecular breeding
SN - 1380-3743
IS - 2
ER -