TY - JOUR

T1 - Living apart together - Bacterial volatiles influence methanotrophic growth and activity

AU - Veraart, A. J.

AU - Garbeva, P.

AU - Van Beersum, F.

AU - Ho, A.

AU - Hordijk, C. A.

AU - Meima-Franke, M.

AU - Zweers, A. J.

AU - Bodelier, P. L.E.

N1 - Funding information: AJV and PLEB were supported by grant 823.001.008 of the Netherlands Organization for Scientific Research, AH by the BE-Basic Program of the Dutch Ministry of Economic Affairs, Agriculture and Innovation. We thank Olav Tyc, Desalegn Etalo, Iris Chardon, Roosmarijn Kobossen, Rosalie Doorn and Max Reumer for help with experiments and data analysis. Data are available from the Dryad Digital Repository: https://doi.org/10.5061/dryad.v31rb, MS-spectra via https://pure.knaw. nl/ at //nioo0039/Lab-Shares-3/GC-QTOF-2/_RAW-DATA/_TD-GC-QTOF/2016/BODELIER/Bodelier.

PY - 2018/4

Y1 - 2018/4

N2 - Volatile organic compounds play an important role in microbial interactions. However, little is known about how volatile-mediated interactions modulate biogeochemical processes. In this study, we show the effect of volatile-mediated interaction on growth and functioning of aerobic methane-oxidizing bacteria, grown in co-culture with five different heterotrophs. Both growth and methane oxidation of Methylobacter luteus were stimulated by interaction with specific heterotrophs. In Methylocystis parvus, we observed significant growth promotion, while methane oxidation was inhibited. Volatolomics of the interaction of each of the methanotrophs with Pseudomonas mandelii, revealed presence of a complex blend of volatiles, including dimethylsulfide, dimethyldisulfide, and bicyclic sesquiterpenes. Although the ecological role of the detected compounds remains to be elucidated, our results provide unprecedented insights into interspecific relations and associated volatiles for stimulating methanotroph functioning, which is of substantial environmental and biotechnological significance.

AB - Volatile organic compounds play an important role in microbial interactions. However, little is known about how volatile-mediated interactions modulate biogeochemical processes. In this study, we show the effect of volatile-mediated interaction on growth and functioning of aerobic methane-oxidizing bacteria, grown in co-culture with five different heterotrophs. Both growth and methane oxidation of Methylobacter luteus were stimulated by interaction with specific heterotrophs. In Methylocystis parvus, we observed significant growth promotion, while methane oxidation was inhibited. Volatolomics of the interaction of each of the methanotrophs with Pseudomonas mandelii, revealed presence of a complex blend of volatiles, including dimethylsulfide, dimethyldisulfide, and bicyclic sesquiterpenes. Although the ecological role of the detected compounds remains to be elucidated, our results provide unprecedented insights into interspecific relations and associated volatiles for stimulating methanotroph functioning, which is of substantial environmental and biotechnological significance.

UR - http://www.scopus.com/inward/record.url?scp=85041219532&partnerID=8YFLogxK

U2 - 10.1038/s41396-018-0055-7

DO - 10.1038/s41396-018-0055-7

M3 - Article

C2 - 29382947

AN - SCOPUS:85041219532

VL - 12

SP - 1163

EP - 1166

JO - ISME Journal

JF - ISME Journal

SN - 1751-7362

IS - 4

ER -