TY - JOUR

T1 - Liquid-Infused Structured Titanium Surfaces

T2 - Antiadhesive Mechanism to Repel Streptococcus oralis Biofilms

AU - Doll, Katharina

AU - Yang, Ines

AU - Fadeeva, Elena

AU - Kommerein, Nadine

AU - Szafrański, Szymon P.

AU - Bei Der Wieden, Gesa

AU - Greuling, Andreas

AU - Winkel, Andreas

AU - Chichkov, Boris N.

AU - Stumpp, Nico S.

AU - Stiesch, Meike

N1 - unding information: The authors would like to thank: Marcus Stollhans and Costenoble GmbH & Co. KG for kindly providing Krytox lubricant; Patrick Lang and Henrik Peisker from Nanosurf AG and Dario Ossola from Cytosurge AG for their substantial support with single bacterial cell force spectroscopy; Marly Dalton and Rainer Schreeb for genome sequencing; Agnes Nielsen, Michael Jarek, Sabin Bhuju and Susanne Häußler from the Helmholtz Center for Infection Research for cDNA synthesis and RNASeq; and Lara Kü hnle, Kerstin Elbert and Ronja Hagemeier for excellent technical assistance.

PY - 2019/7/3

Y1 - 2019/7/3

N2 - To combat implant-associated infections, there is a need for novel materials which effectively inhibit bacterial biofilm formation. In the present study, the antiadhesive properties of titanium surface functionalization based on the "slippery liquid-infused porous surfaces" (SLIPS) principle were demonstrated and the underlying mechanism was analyzed. The immobilized liquid layer was stable over 13 days of continuous flow in an oral flow chamber system. With increasing flow rates, the surface exhibited a significant reduction in attached biofilm of both the oral initial colonizer Streptococcus oralis and an oral multispecies biofilm composed of S. oralis, Actinomyces naeslundii, Veillonella dispar, and Porphyromonas gingivalis. Using single cell force spectroscopy, reduced S. oralis adhesion forces on the lubricant layer could be measured. Gene expression patterns in biofilms on SLIPS, on control surfaces, and expression patterns of planktonic cultures were also compared. For this purpose, the genome of S. oralis strain ATCC 9811 was sequenced using PacBio Sequel technology. Even though biofilm cells showed clear changes in gene expression compared to planktonic cells, no differences could be detected between bacteria on SLIPS and on control surfaces. Therefore, it can be concluded that the ability of liquid-infused titanium to repel S. oralis biofilms is mainly due to weakened bacterial adhesion to the underlying liquid interface.

AB - To combat implant-associated infections, there is a need for novel materials which effectively inhibit bacterial biofilm formation. In the present study, the antiadhesive properties of titanium surface functionalization based on the "slippery liquid-infused porous surfaces" (SLIPS) principle were demonstrated and the underlying mechanism was analyzed. The immobilized liquid layer was stable over 13 days of continuous flow in an oral flow chamber system. With increasing flow rates, the surface exhibited a significant reduction in attached biofilm of both the oral initial colonizer Streptococcus oralis and an oral multispecies biofilm composed of S. oralis, Actinomyces naeslundii, Veillonella dispar, and Porphyromonas gingivalis. Using single cell force spectroscopy, reduced S. oralis adhesion forces on the lubricant layer could be measured. Gene expression patterns in biofilms on SLIPS, on control surfaces, and expression patterns of planktonic cultures were also compared. For this purpose, the genome of S. oralis strain ATCC 9811 was sequenced using PacBio Sequel technology. Even though biofilm cells showed clear changes in gene expression compared to planktonic cells, no differences could be detected between bacteria on SLIPS and on control surfaces. Therefore, it can be concluded that the ability of liquid-infused titanium to repel S. oralis biofilms is mainly due to weakened bacterial adhesion to the underlying liquid interface.

KW - bacterial adhesion force

KW - FluidFM

KW - oral multispecies biofilm

KW - RNASeq

KW - Slippery liquid-infused porous surface

KW - Streptococcus oralis genome sequencing

UR - http://www.scopus.com/inward/record.url?scp=85068119081&partnerID=8YFLogxK

U2 - 10.1021/acsami.9b06817

DO - 10.1021/acsami.9b06817

M3 - Article

C2 - 31173692

AN - SCOPUS:85068119081

VL - 11

SP - 23026

EP - 23038

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

SN - 1944-8244

IS - 26

ER -