Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 6536-6550 |
| Seitenumfang | 15 |
| Fachzeitschrift | Biomedical Optics Express |
| Jahrgang | 11 |
| Ausgabenummer | 11 |
| Frühes Online-Datum | 19 Okt. 2020 |
| Publikationsstatus | Veröffentlicht - 1 Nov. 2020 |
Abstract
Light as a tool in medical therapy and biological research has been studied extensively and its application is subject to continuous improvement. However, safe and efficient application of light-based methods in photomedicine or optogenetics requires knowledge about the optical properties of the target tissue as well as the response characteristics of the stimulated cells. Here, we used tissue phantoms and a heart-like light-sensitive cell line to investigate optogenetic stimulation through tissue layers. The input power necessary for successful stimulation could be described as a function of phantom thickness. A model of light transmission through the tissue phantoms gives insights into the expected stimulation efficiency. Cell-type specific effects are identified that result in deviations of the stimulation threshold from the modelled predictions. This study provides insights into the complex interplay between light, tissue and cells during deep-tissue optogenetics. It can serve as an orientation for safe implementation of light-based methods in vivo.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Physik und Astronomie (insg.)
- Atom- und Molekularphysik sowie Optik
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in: Biomedical Optics Express, Jahrgang 11, Nr. 11, 01.11.2020, S. 6536-6550.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Light-cell interactions in depth-resolved optogenetics
AU - Johannsmeier, Sonja
AU - Wenzel, Johannes
AU - Torres-Mapa, Maria L.
AU - Junge, Sebastian
AU - Sasse, Philipp
AU - Stockhausen, Joshua D.
AU - Ripken, Tammo
AU - Heinemann, Dag
AU - Heisterkamp, Alexander
N1 - Funding Information: Deutsche Forschungsgemeinschaft (380524518/SA1785/9-1, Cluster of Excellence "Hearing4all", EXC 1077/1); Bundesministerium für Bildung und Forschung (13N14085, funding program Photonics Research Germany, project BioPACE).
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Light as a tool in medical therapy and biological research has been studied extensively and its application is subject to continuous improvement. However, safe and efficient application of light-based methods in photomedicine or optogenetics requires knowledge about the optical properties of the target tissue as well as the response characteristics of the stimulated cells. Here, we used tissue phantoms and a heart-like light-sensitive cell line to investigate optogenetic stimulation through tissue layers. The input power necessary for successful stimulation could be described as a function of phantom thickness. A model of light transmission through the tissue phantoms gives insights into the expected stimulation efficiency. Cell-type specific effects are identified that result in deviations of the stimulation threshold from the modelled predictions. This study provides insights into the complex interplay between light, tissue and cells during deep-tissue optogenetics. It can serve as an orientation for safe implementation of light-based methods in vivo.
AB - Light as a tool in medical therapy and biological research has been studied extensively and its application is subject to continuous improvement. However, safe and efficient application of light-based methods in photomedicine or optogenetics requires knowledge about the optical properties of the target tissue as well as the response characteristics of the stimulated cells. Here, we used tissue phantoms and a heart-like light-sensitive cell line to investigate optogenetic stimulation through tissue layers. The input power necessary for successful stimulation could be described as a function of phantom thickness. A model of light transmission through the tissue phantoms gives insights into the expected stimulation efficiency. Cell-type specific effects are identified that result in deviations of the stimulation threshold from the modelled predictions. This study provides insights into the complex interplay between light, tissue and cells during deep-tissue optogenetics. It can serve as an orientation for safe implementation of light-based methods in vivo.
UR - http://www.scopus.com/inward/record.url?scp=85094965048&partnerID=8YFLogxK
U2 - 10.1364/BOE.404388
DO - 10.1364/BOE.404388
M3 - Article
AN - SCOPUS:85094965048
VL - 11
SP - 6536
EP - 6550
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 11
ER -