L-Galactono-1,4-lactone dehydrogenase (GLDH) Forms Part of Three Subcomplexes of Mitochondrial Complex I in Arabidopsis thaliana

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  • Universidad Nacional de La Plata
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OriginalspracheEnglisch
Seiten (von - bis)14412-14419
Seitenumfang8
FachzeitschriftJournal of Biological Chemistry
Jahrgang287
Ausgabenummer18
PublikationsstatusVeröffentlicht - 29 Feb. 2012

Abstract

L-Galactono-1,4-lactone dehydrogenase (GLDH) catalyzes the terminal step of the Smirnoff-Wheeler pathway for vitamin C (L-ascorbate) biosynthesis in plants. A GLDH in gel activity assay was developed to biochemically investigate GLDH localization in plant mitochondria. It previously has been shown that GLDH forms part of an 850-kDa complex that represents a minor form of the respiratory NADH dehydrogenase complex (complex I). Because accumulation of complex I is disturbed in the absence of GLDH, a role of this enzyme in complex I assembly has been proposed. Here we report that GLDH is associated with two further protein complexes. Using native gel electrophoresis procedures in combination with the in gel GLDH activity assay and immunoblotting, two mitochondrial complexes of 470 and 420 kDa were identified. Both complexes are of very low abundance. Protein identifications by mass spectrometry revealed that they include subunits of complex I. Finally, the 850-kDa complex was further investigated and shown to include the complete "peripheral arm" of complex I. GLDH is attached to a membrane domain, which represents a major fragment of the "membrane arm" of complex I. Taken together, our data further support a role of GLDH during complex I formation, which is based on its binding to specific assembly intermediates.

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L-Galactono-1,4-lactone dehydrogenase (GLDH) Forms Part of Three Subcomplexes of Mitochondrial Complex I in Arabidopsis thaliana. / Schertl, Peter; Sunderhaus, Stephanie; Klodmann, Jennifer et al.
in: Journal of Biological Chemistry, Jahrgang 287, Nr. 18, 29.02.2012, S. 14412-14419.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Schertl P, Sunderhaus S, Klodmann J, Gergoff Grozeff GE, Bartoli CG, Braun HP. L-Galactono-1,4-lactone dehydrogenase (GLDH) Forms Part of Three Subcomplexes of Mitochondrial Complex I in Arabidopsis thaliana. Journal of Biological Chemistry. 2012 Feb 29;287(18):14412-14419. doi: 10.1074/jbc.M111.305144, 10.15488/11687
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AU - Sunderhaus, Stephanie

AU - Klodmann, Jennifer

AU - Gergoff Grozeff, Gustavo E.

AU - Bartoli, Carlos G.

AU - Braun, Hans Peter

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