Laser printing of cells into 3D scaffolds

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Autorschaft

  • A. Ovsianikov
  • M. Gruene
  • M. Pflaum
  • L. Koch
  • F. Maiorana
  • M. Wilhelmi
  • A. Haverich
  • B. Chichkov

Externe Organisationen

  • Laser Zentrum Hannover e.V. (LZH)
  • Medizinische Hochschule Hannover (MHH)
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Aufsatznummer014104
FachzeitschriftBIOFABRICATION
Jahrgang2
Ausgabenummer1
PublikationsstatusVeröffentlicht - 10 März 2010
Extern publiziertJa

Abstract

One of the most promising approaches in tissue engineering is the application of 3D scaffolds, which provide cell support and guidance in the initial tissue formation stage. The porosity of the scaffold and internal pore organization influence cell migration and play a major role in its biodegradation dynamics, nutrient diffusion and mechanical stability. In order to control cell migration and cellular interactions within the scaffold, novel technologies capable of producing 3D structures in accordance with predefined design are required. The two-photon polymerization (2PP) technique, used in this report for the fabrication of scaffolds, allows the realization of arbitrary 3D structures with submicron spatial resolution. Highly porous 3D scaffolds, produced by 2PP of acrylated poly(ethylene glycol), are seeded with cells by means of laser-induced forward transfer (LIFT). In this laser printing approach, a propulsive force, resulting from laser-induced shock wave, is used to propel individual cells or cell groups from a donor substrate towards the receiver substrate. We demonstrate that with this technique printing of multiple cell types into 3D scaffolds is possible. Combination of LIFT and 2PP provides a route for the realization of 3D multicellular tissue constructs and artificial ECM engineered on the microscale.

ASJC Scopus Sachgebiete

Zitieren

Laser printing of cells into 3D scaffolds. / Ovsianikov, A.; Gruene, M.; Pflaum, M. et al.
in: BIOFABRICATION, Jahrgang 2, Nr. 1, 014104, 10.03.2010.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Ovsianikov, A, Gruene, M, Pflaum, M, Koch, L, Maiorana, F, Wilhelmi, M, Haverich, A & Chichkov, B 2010, 'Laser printing of cells into 3D scaffolds', BIOFABRICATION, Jg. 2, Nr. 1, 014104. https://doi.org/10.1088/1758-5082/2/1/014104
Ovsianikov, A., Gruene, M., Pflaum, M., Koch, L., Maiorana, F., Wilhelmi, M., Haverich, A., & Chichkov, B. (2010). Laser printing of cells into 3D scaffolds. BIOFABRICATION, 2(1), Artikel 014104. https://doi.org/10.1088/1758-5082/2/1/014104
Ovsianikov A, Gruene M, Pflaum M, Koch L, Maiorana F, Wilhelmi M et al. Laser printing of cells into 3D scaffolds. BIOFABRICATION. 2010 Mär 10;2(1):014104. doi: 10.1088/1758-5082/2/1/014104
Ovsianikov, A. ; Gruene, M. ; Pflaum, M. et al. / Laser printing of cells into 3D scaffolds. in: BIOFABRICATION. 2010 ; Jahrgang 2, Nr. 1.
Download
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AU - Gruene, M.

AU - Pflaum, M.

AU - Koch, L.

AU - Maiorana, F.

AU - Wilhelmi, M.

AU - Haverich, A.

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