Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 065604 |
| Fachzeitschrift | Laser physics letters |
| Jahrgang | 15 |
| Ausgabenummer | 6 |
| Publikationsstatus | Veröffentlicht - Juni 2018 |
Abstract
A technology of laser engineering of microbial systems (LEMS) based on the method of laser-induced transfer of heterogeneous mixtures containing microorganisms (laser bioprinting) is described. This technology involves laser printing of soil microparticles by focusing near-infrared laser pulses on a specially prepared gel/soil mixture spread onto a gold-coated glass plate. The optimal range of laser energies from the point of view of the formation of stable jets and droplets with minimal negative impact on living systems of giant accelerations, laser pulse irradiation, and Au nanoparticles was found. Microsamples of soil were printed on glucose-peptone-yeast agar plates to estimate the LEMS process influence on structural and morphological microbial diversity. The obtained results were compared with traditionally treated soil samples. It was shown that LEMS technology allows significantly increasing the biodiversity of printed organisms and is effective for isolating rare or unculturable microorganisms.
ASJC Scopus Sachgebiete
- Physik und Astronomie (insg.)
- Instrumentierung
- Physik und Astronomie (insg.)
- Physik und Astronomie (sonstige)
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in: Laser physics letters, Jahrgang 15, Nr. 6, 065604, 06.2018.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Laser engineering of microbial systems
AU - Yusupov, V. I.
AU - Gorlenko, M. V.
AU - Cheptsov, V. S.
AU - Minaev, N. V.
AU - Churbanova, E. S.
AU - Zhigarkov, V. S.
AU - Chutko, E. A.
AU - Evlashin, S. A.
AU - Chichkov, B. N.
AU - Bagratashvili, V. N.
N1 - Funding information: This work was supported by the Russian Foundation for Basic Research, Grant No. 16-02-00955 (in the microbiological studies); the Russian Science Foundation, Grant No. 18-15-00277 (in the development of the laser bioprinting technique); the Federal Agency of Scientific Organizations, Agreement No. 007-GZ/C3363/26 (in the development of a new setup for 3D laser printing technologies).
PY - 2018/6
Y1 - 2018/6
N2 - A technology of laser engineering of microbial systems (LEMS) based on the method of laser-induced transfer of heterogeneous mixtures containing microorganisms (laser bioprinting) is described. This technology involves laser printing of soil microparticles by focusing near-infrared laser pulses on a specially prepared gel/soil mixture spread onto a gold-coated glass plate. The optimal range of laser energies from the point of view of the formation of stable jets and droplets with minimal negative impact on living systems of giant accelerations, laser pulse irradiation, and Au nanoparticles was found. Microsamples of soil were printed on glucose-peptone-yeast agar plates to estimate the LEMS process influence on structural and morphological microbial diversity. The obtained results were compared with traditionally treated soil samples. It was shown that LEMS technology allows significantly increasing the biodiversity of printed organisms and is effective for isolating rare or unculturable microorganisms.
AB - A technology of laser engineering of microbial systems (LEMS) based on the method of laser-induced transfer of heterogeneous mixtures containing microorganisms (laser bioprinting) is described. This technology involves laser printing of soil microparticles by focusing near-infrared laser pulses on a specially prepared gel/soil mixture spread onto a gold-coated glass plate. The optimal range of laser energies from the point of view of the formation of stable jets and droplets with minimal negative impact on living systems of giant accelerations, laser pulse irradiation, and Au nanoparticles was found. Microsamples of soil were printed on glucose-peptone-yeast agar plates to estimate the LEMS process influence on structural and morphological microbial diversity. The obtained results were compared with traditionally treated soil samples. It was shown that LEMS technology allows significantly increasing the biodiversity of printed organisms and is effective for isolating rare or unculturable microorganisms.
KW - biodiversity
KW - gel microdroplet
KW - jet acceleration
KW - laser bioprinting
KW - microbe isolation
KW - nanoparticles
KW - unculturable
UR - http://www.scopus.com/inward/record.url?scp=85047127481&partnerID=8YFLogxK
U2 - 10.1088/1612-202x/aab5ef
DO - 10.1088/1612-202x/aab5ef
M3 - Article
AN - SCOPUS:85047127481
VL - 15
JO - Laser physics letters
JF - Laser physics letters
SN - 1612-2011
IS - 6
M1 - 065604
ER -