Isolation of a potassium-selective ion channel from the plasma membrane of the broad bean Vicia faba L

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

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  • Carsten Zeilinger

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OriginalspracheEnglisch
Seiten (von - bis)278-82
Seitenumfang5
FachzeitschriftFEBS letters
Jahrgang348
Ausgabenummer3
PublikationsstatusVeröffentlicht - 18 Juli 1994

Abstract

Potassium ions are essential for plant nutrition and play an important role in osmoregulation and plant movement. Recent patch clamp investigations and molecular analysis have identified voltage-dependent potassium channels responsible for translocation of this ion across plant membranes. In order to characterize these transporters on the biochemical level, a potassium channel protein was isolated from Vicia faba under preservation of its functional integrity. Potassium channel activity was monitored by tracer flux and black lipid bilayer measurements. The capability for K+ transport copurified with a 67 kDa protein from V. faba plasma membranes. A monoclonal antibody directed against the 67 kDa protein blocked channel activity. Single channels with a unit conductance of 40 pS were highly selective for K+ over Na+.

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Isolation of a potassium-selective ion channel from the plasma membrane of the broad bean Vicia faba L. / Zeilinger, Carsten.
in: FEBS letters, Jahrgang 348, Nr. 3, 18.07.1994, S. 278-82.

Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review

Zeilinger C. Isolation of a potassium-selective ion channel from the plasma membrane of the broad bean Vicia faba L. FEBS letters. 1994 Jul 18;348(3):278-82. doi: 10.1016/0014-5793(94)00629-6
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KW - Rubidium Radioisotopes

KW - Tetraethylammonium

KW - Tetraethylammonium Compounds/pharmacology

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