Investigation of the Electrokinetic Properties of HIV-Based Virus-Like Particles

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Tobias Wolf
  • Christoph Grau
  • Jamila Franca Rosengarten
  • Jörn Stitz
  • Jan Wilkens
  • Stéphan Barbe

Organisationseinheiten

Externe Organisationen

  • Technische Hochschule Köln
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Details

OriginalspracheEnglisch
Seiten (von - bis)4762-4771
Seitenumfang10
FachzeitschriftLANGMUIR
Jahrgang40
Ausgabenummer9
Frühes Online-Datum22 Feb. 2024
PublikationsstatusVeröffentlicht - 5 März 2024

Abstract

The antigen density on the surface of HIV-based virus-like particles (VLPs) plays a crucial role in the improvement of HIV vaccine potency. HIV VLPs consist of a dense protein core, which is surrounded by a lipid bilayer and whose surface is usually decorated with antigenic glycoproteins. The successful downstream processing of these particles is challenging, and the high-resolution and cost-efficient purification of HIV-based VLPs has not yet been achieved. Chromatography, one of the major unit operations involved in HIV VLP purification strategies, is usually carried out by means of ion exchangers or ion-exchange membranes. Understanding the electrokinetic behavior of HIV-based VLPs may help to improve the adjustment and efficiency of the corresponding chromatographic processes. In this study, we investigated the electrokinetics and aggregation of both undecorated and decorated VLPs and interpreted the data from the perspective of the soft particle model developed by Ohshima (OSPM), which fails to fully predict the behavior of the studied VLPs. Post-Ohshima literature, and particularly the soft multilayer particle model developed by Langlet et al., provides an alternative theoretical framework to overcome the limits of the OSPM. We finally hypothesized that the electrophoretic mobility of HIV-based VLPs is controlled by an electrohydrodynamic interplay between envelope glycoproteins, lipid bilayer, and Gag envelope.

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Investigation of the Electrokinetic Properties of HIV-Based Virus-Like Particles. / Wolf, Tobias; Grau, Christoph; Rosengarten, Jamila Franca et al.
in: LANGMUIR, Jahrgang 40, Nr. 9, 05.03.2024, S. 4762-4771.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Wolf, T, Grau, C, Rosengarten, JF, Stitz, J, Wilkens, J & Barbe, S 2024, 'Investigation of the Electrokinetic Properties of HIV-Based Virus-Like Particles', LANGMUIR, Jg. 40, Nr. 9, S. 4762-4771. https://doi.org/10.1021/acs.langmuir.3c03535
Wolf, T., Grau, C., Rosengarten, J. F., Stitz, J., Wilkens, J., & Barbe, S. (2024). Investigation of the Electrokinetic Properties of HIV-Based Virus-Like Particles. LANGMUIR, 40(9), 4762-4771. https://doi.org/10.1021/acs.langmuir.3c03535
Wolf T, Grau C, Rosengarten JF, Stitz J, Wilkens J, Barbe S. Investigation of the Electrokinetic Properties of HIV-Based Virus-Like Particles. LANGMUIR. 2024 Mär 5;40(9):4762-4771. Epub 2024 Feb 22. doi: 10.1021/acs.langmuir.3c03535
Wolf, Tobias ; Grau, Christoph ; Rosengarten, Jamila Franca et al. / Investigation of the Electrokinetic Properties of HIV-Based Virus-Like Particles. in: LANGMUIR. 2024 ; Jahrgang 40, Nr. 9. S. 4762-4771.
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AU - Stitz, Jörn

AU - Wilkens, Jan

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N1 - Funding Information: The authors thank Beatrix Martiny and Astrid Schauss at the CECAD Cologne Imaging Facility for excellent sample preparation and electron microscopic-analysis of VLPs. This work was supported by grants from the German Federal Ministry of Education and Research, funding program Forschung an Fachhochschulen, contract numbers 13FH767IA6 to SB and JS.

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