TY - JOUR

T1 - Investigation of low-level 242Pu contamination on nutrition disturbance and oxidative stress in Solanum tuberosum L

AU - Gupta, Dharmendra K.

AU - Tawussi, Frank

AU - Hölzer, Alex

AU - Hamann, Linda

AU - Walther, Clemens

N1 - Funding information: We acknowledge financial support from the BMBF for FT (funding 02S9082A) and DG (funding 02S9276D). AH thanks the Sasse foundation for their continuous support.

PY - 2017/5/23

Y1 - 2017/5/23

N2 - Plutonium associated with higher molecular weight molecules is presumed to be poorly mobile and hardly plant available. In our present study, we investigate the uptake and effects of Pu treatments on Solanum tuberosum plants in amended Hoagland medium at concentrations of [242Pu] = 100 and 500 nm, respectively. We found a direct proof of oxidative stress in the plants caused by these rather low concentrations. For the confirmation of oxidative stress, we explored the production of nitric oxide (NO) and hydrogen peroxide (H2O2) by epifluorescence microscopy. Oxidative stress markers like lipid peroxidation and superoxide radicals (O2 •−) are monitored through histochemical analysis. The biochemical parameters i.e. chlorophyll and carotenoids are measured as an indicator of cellular damage in the tested plants including the enzymatic parameters such as catalase and glutathione reductase. From our work, we conclude that Pu in low concentration has no significant effects on the uptake of many trace and macroelements. In contrast, the content of O2 •−, malondialdehyde (MDA), and H2O2 increases with increasing Pu concentration in the solution, while the opposite effects was found for NO, catalase, and glutathione reductase. These findings prove that even low concentration of Pu regulates ROS production and generate oxidative stress in S. tuberosum L.

AB - Plutonium associated with higher molecular weight molecules is presumed to be poorly mobile and hardly plant available. In our present study, we investigate the uptake and effects of Pu treatments on Solanum tuberosum plants in amended Hoagland medium at concentrations of [242Pu] = 100 and 500 nm, respectively. We found a direct proof of oxidative stress in the plants caused by these rather low concentrations. For the confirmation of oxidative stress, we explored the production of nitric oxide (NO) and hydrogen peroxide (H2O2) by epifluorescence microscopy. Oxidative stress markers like lipid peroxidation and superoxide radicals (O2 •−) are monitored through histochemical analysis. The biochemical parameters i.e. chlorophyll and carotenoids are measured as an indicator of cellular damage in the tested plants including the enzymatic parameters such as catalase and glutathione reductase. From our work, we conclude that Pu in low concentration has no significant effects on the uptake of many trace and macroelements. In contrast, the content of O2 •−, malondialdehyde (MDA), and H2O2 increases with increasing Pu concentration in the solution, while the opposite effects was found for NO, catalase, and glutathione reductase. These findings prove that even low concentration of Pu regulates ROS production and generate oxidative stress in S. tuberosum L.

KW - Catalase

KW - Glutathione reductase

KW - Hydrogen peroxide

KW - Lipid peroxidation

KW - Macro/microelements

KW - Nitric oxide

KW - Plutonium

UR - http://www.scopus.com/inward/record.url?scp=85019889215&partnerID=8YFLogxK

U2 - 10.1007/s11356-017-9071-9

DO - 10.1007/s11356-017-9071-9

M3 - Article

C2 - 28537023

AN - SCOPUS:85019889215

VL - 24

SP - 16050

EP - 16061

JO - Environmental Science and Pollution Research

JF - Environmental Science and Pollution Research

SN - 0944-1344

IS - 19

ER -