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Investigating Ultrafiltration Membranes and Operation Modes for Improved Lentiviral Vector Processing

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autorschaft

  • Jennifer J. Labisch
  • Maria Evangelopoulou
  • Tobias Schleuß
  • Andreas Pickl

Organisationseinheiten

Externe Organisationen

  • Sartorius AG

Details

OriginalspracheEnglisch
Aufsatznummere202400057
Seitenumfang12
FachzeitschriftEngineering in life sciences
Jahrgang25
Ausgabenummer1
PublikationsstatusVeröffentlicht - 9 Jan. 2025

Abstract

The demand for lentiviral vectors (LVs) as tools for ex vivo gene therapies is ever-increasing. Despite their promising applications, challenges in LV production remain largely due to the fragile envelope, which challenges the maintenance of vector stability. Thus, downstream processing optimization to enhance efficiency, yield, and product quality is necessary. This study investigated the influence of membrane types and filtration devices during ultrafiltration (UF). Nine different membrane materials consisting of polyethersulfone (PES), regenerated cellulose, or Hydrosart, with distinct molecular weight cutoffs, were evaluated in stirred cells, centrifugal ultrafilters, and crossflow cassettes. The evaluation was based on the ability to retain infectious LV particles and remove impurities. The analysis revealed that a reinforced 100 kDa PES and a 300 kDa Hydrosart membrane had the best overall ability to concentrate infectious LVs and remove DNA, especially when operated in a stirred cell. Challenges were seen in the nonoptimized crossflow cassette process, where infectious LV recovery was generally lower compared to other devices. We demonstrated that membrane material and filtration device have a direct impact on the efficiency of LV UF.

ASJC Scopus Sachgebiete

Zitieren

Investigating Ultrafiltration Membranes and Operation Modes for Improved Lentiviral Vector Processing. / Labisch, Jennifer J.; Evangelopoulou, Maria; Schleuß, Tobias et al.
in: Engineering in life sciences, Jahrgang 25, Nr. 1, e202400057, 09.01.2025.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Labisch JJ, Evangelopoulou M, Schleuß T, Pickl A. Investigating Ultrafiltration Membranes and Operation Modes for Improved Lentiviral Vector Processing. Engineering in life sciences. 2025 Jan 9;25(1):e202400057. doi: 10.1002/elsc.202400057
Labisch, Jennifer J. ; Evangelopoulou, Maria ; Schleuß, Tobias et al. / Investigating Ultrafiltration Membranes and Operation Modes for Improved Lentiviral Vector Processing. in: Engineering in life sciences. 2025 ; Jahrgang 25, Nr. 1.
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AU - Schleuß, Tobias

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