Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 4935-4944 |
| Seitenumfang | 10 |
| Fachzeitschrift | Analytical methods |
| Jahrgang | 10 |
| Ausgabenummer | 40 |
| Frühes Online-Datum | 25 Sept. 2018 |
| Publikationsstatus | Veröffentlicht - 28 Okt. 2018 |
Abstract
Introduction: Several eicosanoids and other oxylipins are important lipid mediators. Reliable quantification in plasma is important to assess the state of disease, action of drugs and the biology of oxylipins. In order to monitor biological changes, low background variability of oxylipin concentrations in biological samples is essential for proper interpretation of oxylipin biology. However, only little is known about the variation in the oxylipin profile in healthy human subjects. Experimental: Inter-day variation in circulating oxylipins after overnight fasting was investigated in healthy young men on either a standardized or non-standardized diet during a (24 to) 48 h time interval. Intra-day variance was investigated during an 8 h time interval (covering breakfast and lunch meals) in men on a standardized diet with blood sampling at 0, 2, 4, 6 and 8 hours. Free oxylipins in plasma were analyzed using a targeted metabolomics platform for the quantification of 160 oxylipins from different precursors. Analytical variation was evaluated based on quality control plasma samples. Results: Free oxylipins in quality control plasma samples showed low variations (<20% for most analytes). Inter-day variations in fasting blood were in the same range, while significant differences were observed within the day (intra-day variance). Conclusion: Based on the low intra-individual inter-day variance in concentrations of free oxylipins, our results demonstrate the suitability of fasting plasma for the investigation of oxylipin biology. In non-fasting plasma samples, the variations were high during the day. Thus, non-fasting plasma samples appear to be unsuitable to evaluate biologically relevant changes, for instance, those caused by disease or drugs. However, it remains to be determined if the same standardized meal results in reproducible modulations of the oxylipin profile allowing evaluation of the oxylipin pattern during the postprandial state.
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- Analytische Chemie
- Chemische Verfahrenstechnik (insg.)
- Allgemeine chemische Verfahrenstechnik
- Ingenieurwesen (insg.)
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in: Analytical methods, Jahrgang 10, Nr. 40, 28.10.2018, S. 4935-4944.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Intra-individual variance of the human plasma oxylipin pattern: low inter-day variability in fasting blood samples versus high variability during the day
AU - Ostermann, Annika I.
AU - Greupner, Theresa
AU - Kutzner, Laura
AU - Hartung, Nicole M.
AU - Hahn, Andreas
AU - Schuchardt, Jan Philipp
AU - Schebb, Nils Helge
N1 - Funding information: This study was supported by grants from the German Research Foundation (Grant SCHE 1801 and SCHU 2516) to NHS and JPS.
PY - 2018/10/28
Y1 - 2018/10/28
N2 - Introduction: Several eicosanoids and other oxylipins are important lipid mediators. Reliable quantification in plasma is important to assess the state of disease, action of drugs and the biology of oxylipins. In order to monitor biological changes, low background variability of oxylipin concentrations in biological samples is essential for proper interpretation of oxylipin biology. However, only little is known about the variation in the oxylipin profile in healthy human subjects. Experimental: Inter-day variation in circulating oxylipins after overnight fasting was investigated in healthy young men on either a standardized or non-standardized diet during a (24 to) 48 h time interval. Intra-day variance was investigated during an 8 h time interval (covering breakfast and lunch meals) in men on a standardized diet with blood sampling at 0, 2, 4, 6 and 8 hours. Free oxylipins in plasma were analyzed using a targeted metabolomics platform for the quantification of 160 oxylipins from different precursors. Analytical variation was evaluated based on quality control plasma samples. Results: Free oxylipins in quality control plasma samples showed low variations (<20% for most analytes). Inter-day variations in fasting blood were in the same range, while significant differences were observed within the day (intra-day variance). Conclusion: Based on the low intra-individual inter-day variance in concentrations of free oxylipins, our results demonstrate the suitability of fasting plasma for the investigation of oxylipin biology. In non-fasting plasma samples, the variations were high during the day. Thus, non-fasting plasma samples appear to be unsuitable to evaluate biologically relevant changes, for instance, those caused by disease or drugs. However, it remains to be determined if the same standardized meal results in reproducible modulations of the oxylipin profile allowing evaluation of the oxylipin pattern during the postprandial state.
AB - Introduction: Several eicosanoids and other oxylipins are important lipid mediators. Reliable quantification in plasma is important to assess the state of disease, action of drugs and the biology of oxylipins. In order to monitor biological changes, low background variability of oxylipin concentrations in biological samples is essential for proper interpretation of oxylipin biology. However, only little is known about the variation in the oxylipin profile in healthy human subjects. Experimental: Inter-day variation in circulating oxylipins after overnight fasting was investigated in healthy young men on either a standardized or non-standardized diet during a (24 to) 48 h time interval. Intra-day variance was investigated during an 8 h time interval (covering breakfast and lunch meals) in men on a standardized diet with blood sampling at 0, 2, 4, 6 and 8 hours. Free oxylipins in plasma were analyzed using a targeted metabolomics platform for the quantification of 160 oxylipins from different precursors. Analytical variation was evaluated based on quality control plasma samples. Results: Free oxylipins in quality control plasma samples showed low variations (<20% for most analytes). Inter-day variations in fasting blood were in the same range, while significant differences were observed within the day (intra-day variance). Conclusion: Based on the low intra-individual inter-day variance in concentrations of free oxylipins, our results demonstrate the suitability of fasting plasma for the investigation of oxylipin biology. In non-fasting plasma samples, the variations were high during the day. Thus, non-fasting plasma samples appear to be unsuitable to evaluate biologically relevant changes, for instance, those caused by disease or drugs. However, it remains to be determined if the same standardized meal results in reproducible modulations of the oxylipin profile allowing evaluation of the oxylipin pattern during the postprandial state.
UR - http://www.scopus.com/inward/record.url?scp=85055288874&partnerID=8YFLogxK
U2 - 10.1039/c8ay01753k
DO - 10.1039/c8ay01753k
M3 - Article
AN - SCOPUS:85055288874
VL - 10
SP - 4935
EP - 4944
JO - Analytical methods
JF - Analytical methods
SN - 1759-9660
IS - 40
ER -