Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 295-298 |
| Seitenumfang | 4 |
| Fachzeitschrift | Plant Cell, Tissue and Organ Culture |
| Jahrgang | 67 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - Dez. 2001 |
Abstract
Efficient protocols have been developed to induce adventitious shoots in different types of explants of Campanula carpatica Jacq. More than five shoots per explant developed on hypocotyls of 5-week-old seedlings after 2 weeks of culture. Hypocotyls produced twice as many shoots as the cotyledons. TDZ proved to be about 6 times more efficient than BA. NAA had to be added to the regeneration medium to obtain the optimal balance of auxin and cytokinin to induce shoot regeneration. Significant differences were noted between different growth regulator concentrations in their effects on shoot organogenesis. BA induced double the number of callus clumps as TDZ. Incubation of explants in the dark produced about 6 shoots per explant while those in the light produced about 2 shoots per explant. Explants derived from 5-week-old seedlings were five times more regenerative compared to those derived from 15-week-old seedlings. Explants from cv. White Uniform were more organogenic than those from cv. Blue Clip. Root segments were also found to form shoots when treated with CPPU.
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in: Plant Cell, Tissue and Organ Culture, Jahrgang 67, Nr. 3, 12.2001, S. 295-298.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Induction of adventitious shoots in vitro in Campanula carpatica
AU - Sriskandarajah, Sridevy
AU - Frello, Stefan
AU - Serek, Margrethe
N1 - Funding Information: The project was funded by the Danish Ministry of Agriculture (93S-2466-Å97-01023) and Danish Campanula Growers: Gartneriet Thoruplund A/S (Odense), Gartneriet PKM ApS (Odense), Gartneriet Lumby-holm A/S (Odense), and Gartneriet Elmegaard ApS (Skælskør).
PY - 2001/12
Y1 - 2001/12
N2 - Efficient protocols have been developed to induce adventitious shoots in different types of explants of Campanula carpatica Jacq. More than five shoots per explant developed on hypocotyls of 5-week-old seedlings after 2 weeks of culture. Hypocotyls produced twice as many shoots as the cotyledons. TDZ proved to be about 6 times more efficient than BA. NAA had to be added to the regeneration medium to obtain the optimal balance of auxin and cytokinin to induce shoot regeneration. Significant differences were noted between different growth regulator concentrations in their effects on shoot organogenesis. BA induced double the number of callus clumps as TDZ. Incubation of explants in the dark produced about 6 shoots per explant while those in the light produced about 2 shoots per explant. Explants derived from 5-week-old seedlings were five times more regenerative compared to those derived from 15-week-old seedlings. Explants from cv. White Uniform were more organogenic than those from cv. Blue Clip. Root segments were also found to form shoots when treated with CPPU.
AB - Efficient protocols have been developed to induce adventitious shoots in different types of explants of Campanula carpatica Jacq. More than five shoots per explant developed on hypocotyls of 5-week-old seedlings after 2 weeks of culture. Hypocotyls produced twice as many shoots as the cotyledons. TDZ proved to be about 6 times more efficient than BA. NAA had to be added to the regeneration medium to obtain the optimal balance of auxin and cytokinin to induce shoot regeneration. Significant differences were noted between different growth regulator concentrations in their effects on shoot organogenesis. BA induced double the number of callus clumps as TDZ. Incubation of explants in the dark produced about 6 shoots per explant while those in the light produced about 2 shoots per explant. Explants derived from 5-week-old seedlings were five times more regenerative compared to those derived from 15-week-old seedlings. Explants from cv. White Uniform were more organogenic than those from cv. Blue Clip. Root segments were also found to form shoots when treated with CPPU.
KW - Campanula
KW - Shoot regeneration
UR - http://www.scopus.com/inward/record.url?scp=0035660914&partnerID=8YFLogxK
U2 - 10.1023/A:1012786818689
DO - 10.1023/A:1012786818689
M3 - Article
AN - SCOPUS:0035660914
VL - 67
SP - 295
EP - 298
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 3
ER -