TY - JOUR

T1 - In-depth analysis of switchable glycerol based polymeric coatings for cell sheet engineering

AU - Becherer, Tobias

AU - Heinen, Silke

AU - Wei, Qiang

AU - Haag, Rainer

AU - Weinhart, Marie

N1 - Funding Information: This work was supported by the Helmholtz Virtual Institute, the Focus Area Nanoscale of Freie Universität Berlin, Poly4Bio (BMBF), and SFB 765 (DFG). We thank Dr. J. Dernedde and Dr. S. Riese for kindly providing NIH 3T3 fibroblasts. Publisher Copyright: © 2015 Acta Materialia Inc.

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Scaffold-free cell sheet engineering using thermoresponsive substrates provides a promising alternative to conventional tissue engineering which in general employs biodegradable scaffold materials. We have previously developed a thermoresponsive coating with glycerol based linear copolymers that enables gentle harvesting of entire cell sheets. In this article we present an in-depth analysis of these thermoresponsive linear polyglycidyl ethers and their performance as coating for substrates in cell culture in comparison with commercially available poly(N-isopropylacrylamide) (PNIPAM) coated culture dishes. A series of copolymers of glycidyl methyl ether (GME) and glycidyl ethyl ether (EGE) was prepared in order to study their thermoresponsive properties in solution and on the surface with respect to the comonomer ratio. In both cases, when grafted to planar surfaces or spherical nanoparticles, the applied thermoresponsive polyglycerol coatings render the respective surfaces switchable. Protein adsorption experiments on copolymer coated planar surfaces with surface plasmon resonance (SPR) spectroscopy reveal the ability of the tested thermoresponsive coatings to be switched between highly protein resistant and adsorptive states. Cell culture experiments demonstrate that these thermoresponsive coatings allow for adhesion and proliferation of NIH 3T3 fibroblasts comparable to TCPS and faster than on PNIPAM substrates. Temperature triggered detachment of complete cell sheets from copolymer coated substrates was accomplished within minutes while maintaining high viability of the harvested cells. Thus such glycerol based copolymers present a promising alternative to PNIPAM as a thermoresponsive coating of cell culture substrates.

AB - Scaffold-free cell sheet engineering using thermoresponsive substrates provides a promising alternative to conventional tissue engineering which in general employs biodegradable scaffold materials. We have previously developed a thermoresponsive coating with glycerol based linear copolymers that enables gentle harvesting of entire cell sheets. In this article we present an in-depth analysis of these thermoresponsive linear polyglycidyl ethers and their performance as coating for substrates in cell culture in comparison with commercially available poly(N-isopropylacrylamide) (PNIPAM) coated culture dishes. A series of copolymers of glycidyl methyl ether (GME) and glycidyl ethyl ether (EGE) was prepared in order to study their thermoresponsive properties in solution and on the surface with respect to the comonomer ratio. In both cases, when grafted to planar surfaces or spherical nanoparticles, the applied thermoresponsive polyglycerol coatings render the respective surfaces switchable. Protein adsorption experiments on copolymer coated planar surfaces with surface plasmon resonance (SPR) spectroscopy reveal the ability of the tested thermoresponsive coatings to be switched between highly protein resistant and adsorptive states. Cell culture experiments demonstrate that these thermoresponsive coatings allow for adhesion and proliferation of NIH 3T3 fibroblasts comparable to TCPS and faster than on PNIPAM substrates. Temperature triggered detachment of complete cell sheets from copolymer coated substrates was accomplished within minutes while maintaining high viability of the harvested cells. Thus such glycerol based copolymers present a promising alternative to PNIPAM as a thermoresponsive coating of cell culture substrates.

KW - Cell sheet engineering

KW - Linear copolymers

KW - Lower critical solution temperature

KW - Polyglycidyl ether

KW - Thermoresponsive surface

UR - http://www.scopus.com/inward/record.url?scp=84940890055&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2015.06.036

DO - 10.1016/j.actbio.2015.06.036

M3 - Article

C2 - 26143602

AN - SCOPUS:84940890055

VL - 25

SP - 43

EP - 55

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

ER -