In vivo Raman spectroscopic and fluorescence study of suspected melanocytic lesions and surrounding healthy skin

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Di Wu
  • Anatoly Fedorov Kukk
  • Rüdiger Panzer
  • Steffen Emmert
  • Bernhard Roth
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Details

OriginalspracheEnglisch
Aufsatznummere202400050
Seitenumfang11
FachzeitschriftJournal of biophotonics
Jahrgang17
Ausgabenummer8
PublikationsstatusVeröffentlicht - 18 Aug. 2024

Abstract

Cutaneous melanoma is the most lethal skin cancer and noninvasively distinguishing it from benign tumor is a major challenge. Raman spectroscopic measurements were conducted on 65 suspected melanocytic lesions and surrounding healthy skin from 47 patients. Compared to the spectra of healthy skin, spectra of melanocytic lesions exhibited lower intensities in carotenoid bands and higher intensities in lipid and melanin bands, suggesting similar variations in the content of these components. Distinct variations were observed among the autofluorescence intensities of healthy skin, benign nevi and malignant melanoma. By incorporating autofluorescence information, the classification accuracy of the support vector machine for spectra of healthy skin, nevi, and melanoma reached 90.2%, surpassing the 87.9% accuracy achieved without autofluorescence, with this difference being statistically significant. These findings indicate the diagnostic value of autofluorescence intensity, which reflect differences in fluorophore content, chemical composition, and structure among healthy skin, nevi, and melanoma.

Zitieren

In vivo Raman spectroscopic and fluorescence study of suspected melanocytic lesions and surrounding healthy skin. / Wu, Di; Fedorov Kukk, Anatoly; Panzer, Rüdiger et al.
in: Journal of biophotonics, Jahrgang 17, Nr. 8, e202400050, 18.08.2024.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Wu D, Fedorov Kukk A, Panzer R, Emmert S, Roth B. In vivo Raman spectroscopic and fluorescence study of suspected melanocytic lesions and surrounding healthy skin. Journal of biophotonics. 2024 Aug 18;17(8):e202400050. doi: 10.1002/jbio.202400050
Wu, Di ; Fedorov Kukk, Anatoly ; Panzer, Rüdiger et al. / In vivo Raman spectroscopic and fluorescence study of suspected melanocytic lesions and surrounding healthy skin. in: Journal of biophotonics. 2024 ; Jahrgang 17, Nr. 8.
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title = "In vivo Raman spectroscopic and fluorescence study of suspected melanocytic lesions and surrounding healthy skin",
abstract = "Cutaneous melanoma is the most lethal skin cancer and noninvasively distinguishing it from benign tumor is a major challenge. Raman spectroscopic measurements were conducted on 65 suspected melanocytic lesions and surrounding healthy skin from 47 patients. Compared to the spectra of healthy skin, spectra of melanocytic lesions exhibited lower intensities in carotenoid bands and higher intensities in lipid and melanin bands, suggesting similar variations in the content of these components. Distinct variations were observed among the autofluorescence intensities of healthy skin, benign nevi and malignant melanoma. By incorporating autofluorescence information, the classification accuracy of the support vector machine for spectra of healthy skin, nevi, and melanoma reached 90.2%, surpassing the 87.9% accuracy achieved without autofluorescence, with this difference being statistically significant. These findings indicate the diagnostic value of autofluorescence intensity, which reflect differences in fluorophore content, chemical composition, and structure among healthy skin, nevi, and melanoma.",
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T1 - In vivo Raman spectroscopic and fluorescence study of suspected melanocytic lesions and surrounding healthy skin

AU - Wu, Di

AU - Fedorov Kukk, Anatoly

AU - Panzer, Rüdiger

AU - Emmert, Steffen

AU - Roth, Bernhard

N1 - Publisher Copyright: © 2024 The Author(s). Journal of Biophotonics published by Wiley-VCH GmbH.

PY - 2024/8/18

Y1 - 2024/8/18

N2 - Cutaneous melanoma is the most lethal skin cancer and noninvasively distinguishing it from benign tumor is a major challenge. Raman spectroscopic measurements were conducted on 65 suspected melanocytic lesions and surrounding healthy skin from 47 patients. Compared to the spectra of healthy skin, spectra of melanocytic lesions exhibited lower intensities in carotenoid bands and higher intensities in lipid and melanin bands, suggesting similar variations in the content of these components. Distinct variations were observed among the autofluorescence intensities of healthy skin, benign nevi and malignant melanoma. By incorporating autofluorescence information, the classification accuracy of the support vector machine for spectra of healthy skin, nevi, and melanoma reached 90.2%, surpassing the 87.9% accuracy achieved without autofluorescence, with this difference being statistically significant. These findings indicate the diagnostic value of autofluorescence intensity, which reflect differences in fluorophore content, chemical composition, and structure among healthy skin, nevi, and melanoma.

AB - Cutaneous melanoma is the most lethal skin cancer and noninvasively distinguishing it from benign tumor is a major challenge. Raman spectroscopic measurements were conducted on 65 suspected melanocytic lesions and surrounding healthy skin from 47 patients. Compared to the spectra of healthy skin, spectra of melanocytic lesions exhibited lower intensities in carotenoid bands and higher intensities in lipid and melanin bands, suggesting similar variations in the content of these components. Distinct variations were observed among the autofluorescence intensities of healthy skin, benign nevi and malignant melanoma. By incorporating autofluorescence information, the classification accuracy of the support vector machine for spectra of healthy skin, nevi, and melanoma reached 90.2%, surpassing the 87.9% accuracy achieved without autofluorescence, with this difference being statistically significant. These findings indicate the diagnostic value of autofluorescence intensity, which reflect differences in fluorophore content, chemical composition, and structure among healthy skin, nevi, and melanoma.

KW - fluorescence

KW - Raman spectroscopy

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