Details
| Originalsprache | Englisch |
|---|---|
| Titel des Sammelwerks | Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV |
| Publikationsstatus | Veröffentlicht - 19 Feb. 2010 |
| Extern publiziert | Ja |
| Veranstaltung | Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV - San Francisco, CA, USA / Vereinigte Staaten Dauer: 25 Jan. 2010 → 27 Jan. 2010 |
Publikationsreihe
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Band | 7554 |
| ISSN (Print) | 1605-7422 |
Abstract
Biocompatibility studies of percutanous implants in animal models usually involve numerous lethal biopsies for subsequent morphometric analysis of the implant-tissue interface. A common drawback of the study protocol is the restriction of the analysis to one final time point. In this study optical coherence tomography (OCT) was used to visualize and enable quantification of the local skin anatomy in the vicinity of a percutaneous implant in an animal model using hairless mice. Non invasive in vivo optical biopsies were taken on predetermined time points after implantation and ex vivo in situ at the day of noticeable inflammation. The custom made Fourier-domain OCT system was programmed for imaging with different scanning schemes. A spoke-pattern of 72 cross-sectional scans which was centred at the midpoint of the circular shaped implants was acquired and worked best for the in-vivo situation. Motion-artefact-free three-dimensional tomograms were obtained from the implant site before excision and preparation for histology. Morphometric parameters such as epithelial downgrowth, distance to normal growth and tissue thickness were extracted from the images with a simple segmentation algorithm. Qualitatively, the OCT B-Scans are in good agreement with histological sections. Therefore, OCT can provide additional valuable information about the implant-tissue interface at freely selectable time points before the lethal biopsy. Locally confined quantitative assessments of tissue-implant interaction for in vivo postoperative monitoring can be carried out.
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Elektronische, optische und magnetische Materialien
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Physik und Astronomie (insg.)
- Atom- und Molekularphysik sowie Optik
- Medizin (insg.)
- Radiologie, Nuklearmedizin und Bildgebung
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Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV. 2010. 75540S (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Band 7554).
Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Aufsatz in Konferenzband › Forschung › Peer-Review
}
TY - GEN
T1 - In vivo optical coherence tomography of percutaneous implants in hairless mice
AU - Donner, S.
AU - Witte, F.
AU - Bartsch, I.
AU - Petraglia, F.
AU - Massow, O.
AU - Heidrich, M.
AU - Lubatschowski, H.
AU - Heisterkamp, Alexander
AU - Krüger, Alexander
PY - 2010/2/19
Y1 - 2010/2/19
N2 - Biocompatibility studies of percutanous implants in animal models usually involve numerous lethal biopsies for subsequent morphometric analysis of the implant-tissue interface. A common drawback of the study protocol is the restriction of the analysis to one final time point. In this study optical coherence tomography (OCT) was used to visualize and enable quantification of the local skin anatomy in the vicinity of a percutaneous implant in an animal model using hairless mice. Non invasive in vivo optical biopsies were taken on predetermined time points after implantation and ex vivo in situ at the day of noticeable inflammation. The custom made Fourier-domain OCT system was programmed for imaging with different scanning schemes. A spoke-pattern of 72 cross-sectional scans which was centred at the midpoint of the circular shaped implants was acquired and worked best for the in-vivo situation. Motion-artefact-free three-dimensional tomograms were obtained from the implant site before excision and preparation for histology. Morphometric parameters such as epithelial downgrowth, distance to normal growth and tissue thickness were extracted from the images with a simple segmentation algorithm. Qualitatively, the OCT B-Scans are in good agreement with histological sections. Therefore, OCT can provide additional valuable information about the implant-tissue interface at freely selectable time points before the lethal biopsy. Locally confined quantitative assessments of tissue-implant interaction for in vivo postoperative monitoring can be carried out.
AB - Biocompatibility studies of percutanous implants in animal models usually involve numerous lethal biopsies for subsequent morphometric analysis of the implant-tissue interface. A common drawback of the study protocol is the restriction of the analysis to one final time point. In this study optical coherence tomography (OCT) was used to visualize and enable quantification of the local skin anatomy in the vicinity of a percutaneous implant in an animal model using hairless mice. Non invasive in vivo optical biopsies were taken on predetermined time points after implantation and ex vivo in situ at the day of noticeable inflammation. The custom made Fourier-domain OCT system was programmed for imaging with different scanning schemes. A spoke-pattern of 72 cross-sectional scans which was centred at the midpoint of the circular shaped implants was acquired and worked best for the in-vivo situation. Motion-artefact-free three-dimensional tomograms were obtained from the implant site before excision and preparation for histology. Morphometric parameters such as epithelial downgrowth, distance to normal growth and tissue thickness were extracted from the images with a simple segmentation algorithm. Qualitatively, the OCT B-Scans are in good agreement with histological sections. Therefore, OCT can provide additional valuable information about the implant-tissue interface at freely selectable time points before the lethal biopsy. Locally confined quantitative assessments of tissue-implant interaction for in vivo postoperative monitoring can be carried out.
KW - Epithelial downgrowth
KW - Implant-tissue interface
KW - Optical Coherence Tomography (OCT)
KW - Percutaneous implants
UR - http://www.scopus.com/inward/record.url?scp=77951582030&partnerID=8YFLogxK
U2 - 10.1117/12.841640
DO - 10.1117/12.841640
M3 - Conference contribution
AN - SCOPUS:77951582030
SN - 9780819479501
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV
T2 - Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV
Y2 - 25 January 2010 through 27 January 2010
ER -