Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 79-87 |
| Seitenumfang | 9 |
| Fachzeitschrift | BioNanoMaterials |
| Jahrgang | 17 |
| Ausgabenummer | 1-2 |
| Publikationsstatus | Veröffentlicht - 19 Apr. 2016 |
Abstract
Wound healing is essential for the restoration of the barrier function of the skin. During this process, cells at the wound edges proliferate and migrate, leading to re-epithelialization of the wound surface. Wound healing assays are used to study the molecular mechanisms of wound repair, as well as in the investigation of potential therapeutics and treatments for improved healing. Numerous models of wound healing have been developed in recent years. In this review, we focus on in vitro assays, as they allow a fast, cost-efficient and ethical alternative to animal models. This paper gives a general overview of 2-dimensional (2D) cell monolayer assays by providing a description of injury methods, as well as an evaluation of each assay's strengths and limitations. We include a section reviewing assays performed in 3-dimensional (3D) culture, which employ bioengineered skin models to capture complex wound healing mechanics like cell-matrix interactions and the interplay of different cell types in the healing process. Finally, we discuss in detail available software tools and algorithms for data analysis.
ASJC Scopus Sachgebiete
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Ingenieurwesen (insg.)
- Biomedizintechnik
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in: BioNanoMaterials, Jahrgang 17, Nr. 1-2, 19.04.2016, S. 79-87.
Publikation: Beitrag in Fachzeitschrift › Übersichtsarbeit › Forschung › Peer-Review
}
TY - JOUR
T1 - In vitro wound healing assays – state of the art
AU - Stamm, Anne
AU - Reimers, Kerstin
AU - Strauß, Sarah
AU - Vogt, Peter
AU - Scheper, Thomas
AU - Pepelanova, Iliyana
PY - 2016/4/19
Y1 - 2016/4/19
N2 - Wound healing is essential for the restoration of the barrier function of the skin. During this process, cells at the wound edges proliferate and migrate, leading to re-epithelialization of the wound surface. Wound healing assays are used to study the molecular mechanisms of wound repair, as well as in the investigation of potential therapeutics and treatments for improved healing. Numerous models of wound healing have been developed in recent years. In this review, we focus on in vitro assays, as they allow a fast, cost-efficient and ethical alternative to animal models. This paper gives a general overview of 2-dimensional (2D) cell monolayer assays by providing a description of injury methods, as well as an evaluation of each assay's strengths and limitations. We include a section reviewing assays performed in 3-dimensional (3D) culture, which employ bioengineered skin models to capture complex wound healing mechanics like cell-matrix interactions and the interplay of different cell types in the healing process. Finally, we discuss in detail available software tools and algorithms for data analysis.
AB - Wound healing is essential for the restoration of the barrier function of the skin. During this process, cells at the wound edges proliferate and migrate, leading to re-epithelialization of the wound surface. Wound healing assays are used to study the molecular mechanisms of wound repair, as well as in the investigation of potential therapeutics and treatments for improved healing. Numerous models of wound healing have been developed in recent years. In this review, we focus on in vitro assays, as they allow a fast, cost-efficient and ethical alternative to animal models. This paper gives a general overview of 2-dimensional (2D) cell monolayer assays by providing a description of injury methods, as well as an evaluation of each assay's strengths and limitations. We include a section reviewing assays performed in 3-dimensional (3D) culture, which employ bioengineered skin models to capture complex wound healing mechanics like cell-matrix interactions and the interplay of different cell types in the healing process. Finally, we discuss in detail available software tools and algorithms for data analysis.
KW - 3D assay
KW - image processing tools
KW - in vitro
KW - scratch assay
KW - skin model
UR - http://www.scopus.com/inward/record.url?scp=84991518476&partnerID=8YFLogxK
U2 - 10.1515/bnm-2016-0002
DO - 10.1515/bnm-2016-0002
M3 - Review article
AN - SCOPUS:84991518476
VL - 17
SP - 79
EP - 87
JO - BioNanoMaterials
JF - BioNanoMaterials
SN - 2193-0651
IS - 1-2
ER -