TY - JOUR

T1 - In vitro propagation of Hippeastrum × chmielii Chm.

T2 - Influence of flurprimidol and the culture in solid or liquid medium and in temporary immersion systems

AU - Ilczuk, Agnieszka

AU - Winkelmann, Traud

AU - Richartz, Simon

AU - Witomska, Maria

AU - Serek, Margrethe

N1 - Funding Information: The authors would like to thank Dr. Katja Saare-Surminski, University of Hamburg, for her advice in the construction of the temporary immersion units, and Dr. Sridevy Sriskandarajah, KVL Copenhagen, for her critical comments on the manuscript. The scholarship of the German Academic Exchange Service (DAAD) and the Polish Ministry of Scientific Research and Information Technology (3P06R 07724) for Agnieszka Ilczuk is gratefully appreciated.

PY - 2005/12

Y1 - 2005/12

N2 - The in vitro propagation of the new hybrid Hippeastrum × chmielii was analysed in this study. Propagation on solid medium, permanent immersion (flasks) and in a temporary immersion system (TIS) using quarters of in vitro grown bulblets as explants was compared. The TIS was constructed as twin-flasks system, the components of which were inexpensive. Within 4 weeks of culture on solid medium 3.9 new bulbs were formed per initial bulb and by using liquid culture systems this number was increased to 6.3-6.5. The number of daily immersions in TIS only slightly influenced the propagation rates and no significant differences to the permanently immersed treatments were observed. By adding flurprimidol (0.1-1.0 mg l-1) to the medium the propagation rate nearly doubled in liquid as well as in solid medium. Moreover, the size of the newly formed bulbs was increased by this inhibitor of gibberellic acid biosynthesis. Flurprimidol strongly reduced the elongation of the leaves while root formation was enhanced.

AB - The in vitro propagation of the new hybrid Hippeastrum × chmielii was analysed in this study. Propagation on solid medium, permanent immersion (flasks) and in a temporary immersion system (TIS) using quarters of in vitro grown bulblets as explants was compared. The TIS was constructed as twin-flasks system, the components of which were inexpensive. Within 4 weeks of culture on solid medium 3.9 new bulbs were formed per initial bulb and by using liquid culture systems this number was increased to 6.3-6.5. The number of daily immersions in TIS only slightly influenced the propagation rates and no significant differences to the permanently immersed treatments were observed. By adding flurprimidol (0.1-1.0 mg l-1) to the medium the propagation rate nearly doubled in liquid as well as in solid medium. Moreover, the size of the newly formed bulbs was increased by this inhibitor of gibberellic acid biosynthesis. Flurprimidol strongly reduced the elongation of the leaves while root formation was enhanced.

KW - Amaryllidaceae

KW - Bulbous ornamentals

KW - Gibberellic acid biosynthesis inhibitor

KW - Growth retardant

UR - http://www.scopus.com/inward/record.url?scp=24744470243&partnerID=8YFLogxK

U2 - 10.1007/s11240-005-8812-5

DO - 10.1007/s11240-005-8812-5

M3 - Article

AN - SCOPUS:24744470243

VL - 83

SP - 339

EP - 346

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

IS - 3

ER -