TY - JOUR

T1 - In situ recovery of the aroma compound perillene from stirred-tank cultured Pleurotus ostreatus using gas stripping and adsorption on polystyrene

AU - Krings, Ulrich

AU - Berger, Ralf G.

N1 - Funding information: Acknowledgement We are grateful for support by the Deutsche Forschungsgemeinschaft (DFG KR 2958/1-1).

PY - 2008/4/15

Y1 - 2008/4/15

N2 - Supplementation of the key metabolite, α-(Z)-acaridiol, to stirred-tank cultured Pleurotus ostreatus was used to demonstrate that integrated in situ product recovery resulted in high conversion rates and quantitative separation of the target product perillene from the nutrient medium. The conversion of β-myrcene by P. ostreatus was scaled-up from shake-flasks into a controlled, stirred tank bioreactor equipped with gas stripping and adsorption on a polystyrene fixed bed. The formation of the attractive flavour compound perillene was measured daily using standard controlled capillary gas chromatography. The formation of α-(Z)-acaridiol was the metabolic bottleneck of the conversion of β-myrcene to perillene. Efficient in situ recovery of the volatile product enabled quantitative separation of the pure flavour compound. Appropriated bioprocessing, i.e. in situ separation of product, steadily shifted the metabolic equilibria and thus accomplished high conversion rate and pure product.

AB - Supplementation of the key metabolite, α-(Z)-acaridiol, to stirred-tank cultured Pleurotus ostreatus was used to demonstrate that integrated in situ product recovery resulted in high conversion rates and quantitative separation of the target product perillene from the nutrient medium. The conversion of β-myrcene by P. ostreatus was scaled-up from shake-flasks into a controlled, stirred tank bioreactor equipped with gas stripping and adsorption on a polystyrene fixed bed. The formation of the attractive flavour compound perillene was measured daily using standard controlled capillary gas chromatography. The formation of α-(Z)-acaridiol was the metabolic bottleneck of the conversion of β-myrcene to perillene. Efficient in situ recovery of the volatile product enabled quantitative separation of the pure flavour compound. Appropriated bioprocessing, i.e. in situ separation of product, steadily shifted the metabolic equilibria and thus accomplished high conversion rate and pure product.

KW - α-(Z)-acaridiol

KW - Adsorption

KW - In situ recovery

KW - Myrcene

KW - Perillene

KW - Pleurotus ostreatus

UR - http://www.scopus.com/inward/record.url?scp=45849137834&partnerID=8YFLogxK

U2 - 10.1007/s10529-008-9690-9

DO - 10.1007/s10529-008-9690-9

M3 - Article

C2 - 18414807

AN - SCOPUS:45849137834

VL - 30

SP - 1347

EP - 1351

JO - Biotechnology letters

JF - Biotechnology letters

SN - 0141-5492

IS - 8

ER -