Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 271 |
| Seitenumfang | 10 |
| Fachzeitschrift | Life |
| Jahrgang | 14 |
| Ausgabenummer | 2 |
| Publikationsstatus | Veröffentlicht - 18 Feb. 2024 |
Abstract
Natural deep eutectic solvents (NADESs) can serve as solvents for enzymes, are biodegradable, and have low toxicities. Eight NADESs with different hydrogen bond acceptors and donors were tested to improve the stability and activity of a lipoxygenase from Basidiomycete Pleurotus sapidus (LOXPSA). Betaine:sorbitol:water (1:1:3, BSorbW) and betaine:ethylene glycol (1:3, BEtGly) had the best impact on the peroxidation of linoleic acid and the side reaction of piperine to the vanilla-like scented compound piperonal. The yield of piperonal in NADESs increased by 43% in BSorbW and 40% in BEtGly compared to the control. The addition of BSorbW also enhanced the enzyme’s stability at various temperatures and increased its activity during incubation at 60 °C. The demonstrated improvement in lipoxygenase activity and stability indicates versatile applications in industry, expanding the potential uses of the enzyme.
ASJC Scopus Sachgebiete
- Agrar- und Biowissenschaften (insg.)
- Ökologie, Evolution, Verhaltenswissenschaften und Systematik
- Biochemie, Genetik und Molekularbiologie (insg.)
- Allgemeine Biochemie, Genetik und Molekularbiologie
- Erdkunde und Planetologie (insg.)
- Astronomie und Planetologie
- Erdkunde und Planetologie (insg.)
- Paläontologie
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in: Life, Jahrgang 14, Nr. 2, 271, 18.02.2024.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Improvement in the Stability and Enzymatic Activity of Pleurotus sapidus Lipoxygenase Dissolved in Natural Deep Eutectic Solvents (NADESs)
AU - Garbe, Maria
AU - Lehmann, Leander Tom
AU - Berger, Ralf Günter
AU - Ersoy, Franziska
N1 - Publisher Copyright: © 2024 by the authors.
PY - 2024/2/18
Y1 - 2024/2/18
N2 - Natural deep eutectic solvents (NADESs) can serve as solvents for enzymes, are biodegradable, and have low toxicities. Eight NADESs with different hydrogen bond acceptors and donors were tested to improve the stability and activity of a lipoxygenase from Basidiomycete Pleurotus sapidus (LOXPSA). Betaine:sorbitol:water (1:1:3, BSorbW) and betaine:ethylene glycol (1:3, BEtGly) had the best impact on the peroxidation of linoleic acid and the side reaction of piperine to the vanilla-like scented compound piperonal. The yield of piperonal in NADESs increased by 43% in BSorbW and 40% in BEtGly compared to the control. The addition of BSorbW also enhanced the enzyme’s stability at various temperatures and increased its activity during incubation at 60 °C. The demonstrated improvement in lipoxygenase activity and stability indicates versatile applications in industry, expanding the potential uses of the enzyme.
AB - Natural deep eutectic solvents (NADESs) can serve as solvents for enzymes, are biodegradable, and have low toxicities. Eight NADESs with different hydrogen bond acceptors and donors were tested to improve the stability and activity of a lipoxygenase from Basidiomycete Pleurotus sapidus (LOXPSA). Betaine:sorbitol:water (1:1:3, BSorbW) and betaine:ethylene glycol (1:3, BEtGly) had the best impact on the peroxidation of linoleic acid and the side reaction of piperine to the vanilla-like scented compound piperonal. The yield of piperonal in NADESs increased by 43% in BSorbW and 40% in BEtGly compared to the control. The addition of BSorbW also enhanced the enzyme’s stability at various temperatures and increased its activity during incubation at 60 °C. The demonstrated improvement in lipoxygenase activity and stability indicates versatile applications in industry, expanding the potential uses of the enzyme.
KW - basidiomycota
KW - lipoxygenase
KW - natural deep eutectic solvent (NADES)
KW - piperonal
KW - Pleurotus sapidus
KW - thermostability
UR - http://www.scopus.com/inward/record.url?scp=85193065352&partnerID=8YFLogxK
U2 - 10.3390/life14020271
DO - 10.3390/life14020271
M3 - Article
AN - SCOPUS:85193065352
VL - 14
JO - Life
JF - Life
IS - 2
M1 - 271
ER -