Improved monoterpene biotransformation with Penicillium sp. by use of a closed gas loop bioreactor

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Michael Pescheck
  • Marco Antonio Mirata
  • Bianca Brauer
  • Ulrich Krings
  • Ralf Günter Berger
  • Jens Schrader

Organisationseinheiten

Externe Organisationen

  • DECHEMA E.V., Karl-Winnacker-Institut
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Details

OriginalspracheEnglisch
Seiten (von - bis)827-836
Seitenumfang10
FachzeitschriftJournal of Industrial Microbiology and Biotechnology
Jahrgang36
Ausgabenummer6
PublikationsstatusVeröffentlicht - 26 März 2009

Abstract

A closed gas loop bioprocess was developed to improve fungal biotransformation of monoterpenes. By circulating monoterpene-saturated process gas, the evaporative loss of the volatile precursor from the medium during the biotransformation was avoided. Penicillium solitum, isolated from kiwi, turned out to be highly tolerant towards monoterpenes and to convert α-pinene to a range of products including verbenone, a valuable aroma compound. The gas loop was mandatory to reproduce the production of 35 mg L-1 verbenone obtained in shake flasks and also in the bioreactor. Penicillium digitatum DSM 62840 regioselectively converted (+)-limonene to the aroma compound α-terpineol, but shake flask cultures revealed a pronounced growth inhibition when initial concentrations exceeded 1.9 mM. In the bioreactor, toxic effects on P. digitatum during biotransformation were alleviated by starting a sequential feeding of non-toxic limonene portions after a preceding growth phase. Closing the precursor-saturated gas loop during the biotransformation allowed for an additional replenishment of limonene via the gas phase. The gas loop system led to a maximum α-terpineol concentration of 1,009 mg L -1 and an average productivity of 8-9 mg L-1 h -1 which represents a doubling of the respective values previously reported. Furthermore, a molar conversion yield of up to 63% was achieved.

ASJC Scopus Sachgebiete

Zitieren

Improved monoterpene biotransformation with Penicillium sp. by use of a closed gas loop bioreactor. / Pescheck, Michael; Mirata, Marco Antonio; Brauer, Bianca et al.
in: Journal of Industrial Microbiology and Biotechnology, Jahrgang 36, Nr. 6, 26.03.2009, S. 827-836.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Pescheck M, Mirata MA, Brauer B, Krings U, Berger RG, Schrader J. Improved monoterpene biotransformation with Penicillium sp. by use of a closed gas loop bioreactor. Journal of Industrial Microbiology and Biotechnology. 2009 Mär 26;36(6):827-836. doi: 10.1007/s10295-009-0558-3
Pescheck, Michael ; Mirata, Marco Antonio ; Brauer, Bianca et al. / Improved monoterpene biotransformation with Penicillium sp. by use of a closed gas loop bioreactor. in: Journal of Industrial Microbiology and Biotechnology. 2009 ; Jahrgang 36, Nr. 6. S. 827-836.
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abstract = "A closed gas loop bioprocess was developed to improve fungal biotransformation of monoterpenes. By circulating monoterpene-saturated process gas, the evaporative loss of the volatile precursor from the medium during the biotransformation was avoided. Penicillium solitum, isolated from kiwi, turned out to be highly tolerant towards monoterpenes and to convert α-pinene to a range of products including verbenone, a valuable aroma compound. The gas loop was mandatory to reproduce the production of 35 mg L-1 verbenone obtained in shake flasks and also in the bioreactor. Penicillium digitatum DSM 62840 regioselectively converted (+)-limonene to the aroma compound α-terpineol, but shake flask cultures revealed a pronounced growth inhibition when initial concentrations exceeded 1.9 mM. In the bioreactor, toxic effects on P. digitatum during biotransformation were alleviated by starting a sequential feeding of non-toxic limonene portions after a preceding growth phase. Closing the precursor-saturated gas loop during the biotransformation allowed for an additional replenishment of limonene via the gas phase. The gas loop system led to a maximum α-terpineol concentration of 1,009 mg L -1 and an average productivity of 8-9 mg L-1 h -1 which represents a doubling of the respective values previously reported. Furthermore, a molar conversion yield of up to 63% was achieved.",
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AU - Pescheck, Michael

AU - Mirata, Marco Antonio

AU - Brauer, Bianca

AU - Krings, Ulrich

AU - Berger, Ralf Günter

AU - Schrader, Jens

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