Improved in vitro rooting of Prunus avium microshoots using a dark treatment and an auxin pulse

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

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Externe Organisationen

  • Institut für Pflanzenkultur e. K.
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Details

OriginalspracheEnglisch
Seiten (von - bis)52-56
Seitenumfang5
FachzeitschriftScientia horticulturae
Jahrgang220
Frühes Online-Datum1 Apr. 2017
PublikationsstatusVeröffentlicht - 16 Juni 2017

Abstract

The influence of a four-day dark treatment and a 48 h IBA pulse on in vitro root formation of Prunus avium microshoots was examined. Both treatments led to significantly higher rooting percentages in the majority of the six genotypes. While the IBA pulse led to a disproportional growth of roots compared to shoots, the plantlets under the dark treatment during root induction were healthy and were not visually distinct from the control plants. Further data collected during the commercial production of 17 genotypes verified the improved root induction using the dark treatment and identified the gelling agent Gelcarin (a carrageenan) as an additional factor with positive effects on rooting. In addition, the importance of in vitro rooting for the successful transfer to greenhouse conditions was confirmed. Rooted plantlets (>3 roots) had a significantly higher survival percentage and showed improved growth during acclimatization compared to unrooted microshoots.

ASJC Scopus Sachgebiete

  • Agrar- und Biowissenschaften (insg.)
  • Gartenbau

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Improved in vitro rooting of Prunus avium microshoots using a dark treatment and an auxin pulse. / Quambusch, Mona; Gruß, Silke; Pscherer, Toni et al.
in: Scientia horticulturae, Jahrgang 220, 16.06.2017, S. 52-56.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Quambusch M, Gruß S, Pscherer T, Winkelmann T, Bartsch M. Improved in vitro rooting of Prunus avium microshoots using a dark treatment and an auxin pulse. Scientia horticulturae. 2017 Jun 16;220:52-56. Epub 2017 Apr 1. doi: 10.1016/j.scienta.2017.03.020
Quambusch, Mona ; Gruß, Silke ; Pscherer, Toni et al. / Improved in vitro rooting of Prunus avium microshoots using a dark treatment and an auxin pulse. in: Scientia horticulturae. 2017 ; Jahrgang 220. S. 52-56.
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abstract = "The influence of a four-day dark treatment and a 48 h IBA pulse on in vitro root formation of Prunus avium microshoots was examined. Both treatments led to significantly higher rooting percentages in the majority of the six genotypes. While the IBA pulse led to a disproportional growth of roots compared to shoots, the plantlets under the dark treatment during root induction were healthy and were not visually distinct from the control plants. Further data collected during the commercial production of 17 genotypes verified the improved root induction using the dark treatment and identified the gelling agent Gelcarin (a carrageenan) as an additional factor with positive effects on rooting. In addition, the importance of in vitro rooting for the successful transfer to greenhouse conditions was confirmed. Rooted plantlets (>3 roots) had a significantly higher survival percentage and showed improved growth during acclimatization compared to unrooted microshoots.",
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AU - Quambusch, Mona

AU - Gruß, Silke

AU - Pscherer, Toni

AU - Winkelmann, Traud

AU - Bartsch, Melanie

N1 - Publisher Copyright: © 2017 Elsevier B.V. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

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N2 - The influence of a four-day dark treatment and a 48 h IBA pulse on in vitro root formation of Prunus avium microshoots was examined. Both treatments led to significantly higher rooting percentages in the majority of the six genotypes. While the IBA pulse led to a disproportional growth of roots compared to shoots, the plantlets under the dark treatment during root induction were healthy and were not visually distinct from the control plants. Further data collected during the commercial production of 17 genotypes verified the improved root induction using the dark treatment and identified the gelling agent Gelcarin (a carrageenan) as an additional factor with positive effects on rooting. In addition, the importance of in vitro rooting for the successful transfer to greenhouse conditions was confirmed. Rooted plantlets (>3 roots) had a significantly higher survival percentage and showed improved growth during acclimatization compared to unrooted microshoots.

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