Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 113-122 |
| Seitenumfang | 10 |
| Fachzeitschrift | Analytica chimica acta |
| Jahrgang | 249 |
| Ausgabenummer | 1 |
| Publikationsstatus | Veröffentlicht - 1991 |
Abstract
A system for real-time monitoring of specific proteins in fermentation processes is introduced. For measurements the turbidity caused by aggregates formed between the proteins to be detected and their antibodies is measured photometrically at 340 nm. The flow-injection analysis principle was used to automate the assay fully, including calibration and washing steps. The assay was intended for on-line product monitoring. It was therefore optimized to cover concentration ranges of 1-1000 mg l-1. The analyser was used to measure monoclonal antibodies (mab) produced in fermentations of mouse-mouse hybridoma cells and to quantify pullulanase isoenzymes produced in a fermentation of Clostridium thermosulfurogenes. The fermentations took between 240 and 450 h, including extended phases of steady-state production. During that rime, the long-term stability of the analyser system was excellent. A relative standard deviation of less than 2% was calculated for the data. A conventional ELISA served as a reference assay in the mab measurement. The enzymatic activity found in comparable off-line samples was used to correlate the on-line pullulanase measurements. Correlation coefficients between 0.94 and 0.99 were found between the on-line assay and the reference assays.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Analytische Chemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Umweltwissenschaften (insg.)
- Umweltchemie
- Chemie (insg.)
- Spektroskopie
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in: Analytica chimica acta, Jahrgang 249, Nr. 1, 1991, S. 113-122.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Immunological on-line detection of specific proteins during fermentation processes
AU - Freitag, Ruth
AU - Fenge, Christel
AU - Scheper, Thomas
AU - Schügerl, Karl
AU - Spreinat, Andreas
AU - Antranikian, Garo
AU - Fraune, Elisabeth
N1 - Funding information: This work was supported by a DECHEMA grant to R. Freitag, grant No. 0318815A from the Bundesministerium ftir Forschung and Technolo-gie, and by the BMFT within the Schwerpunkt: Grundlagen der Bioprozesstechnik.
PY - 1991
Y1 - 1991
N2 - A system for real-time monitoring of specific proteins in fermentation processes is introduced. For measurements the turbidity caused by aggregates formed between the proteins to be detected and their antibodies is measured photometrically at 340 nm. The flow-injection analysis principle was used to automate the assay fully, including calibration and washing steps. The assay was intended for on-line product monitoring. It was therefore optimized to cover concentration ranges of 1-1000 mg l-1. The analyser was used to measure monoclonal antibodies (mab) produced in fermentations of mouse-mouse hybridoma cells and to quantify pullulanase isoenzymes produced in a fermentation of Clostridium thermosulfurogenes. The fermentations took between 240 and 450 h, including extended phases of steady-state production. During that rime, the long-term stability of the analyser system was excellent. A relative standard deviation of less than 2% was calculated for the data. A conventional ELISA served as a reference assay in the mab measurement. The enzymatic activity found in comparable off-line samples was used to correlate the on-line pullulanase measurements. Correlation coefficients between 0.94 and 0.99 were found between the on-line assay and the reference assays.
AB - A system for real-time monitoring of specific proteins in fermentation processes is introduced. For measurements the turbidity caused by aggregates formed between the proteins to be detected and their antibodies is measured photometrically at 340 nm. The flow-injection analysis principle was used to automate the assay fully, including calibration and washing steps. The assay was intended for on-line product monitoring. It was therefore optimized to cover concentration ranges of 1-1000 mg l-1. The analyser was used to measure monoclonal antibodies (mab) produced in fermentations of mouse-mouse hybridoma cells and to quantify pullulanase isoenzymes produced in a fermentation of Clostridium thermosulfurogenes. The fermentations took between 240 and 450 h, including extended phases of steady-state production. During that rime, the long-term stability of the analyser system was excellent. A relative standard deviation of less than 2% was calculated for the data. A conventional ELISA served as a reference assay in the mab measurement. The enzymatic activity found in comparable off-line samples was used to correlate the on-line pullulanase measurements. Correlation coefficients between 0.94 and 0.99 were found between the on-line assay and the reference assays.
KW - Bioprocess monitoring
KW - Fermentation
KW - Flow system
KW - Process analysis
KW - Proteins
KW - Turbidimetry
UR - http://www.scopus.com/inward/record.url?scp=0025833670&partnerID=8YFLogxK
U2 - 10.1016/0003-2670(91)87014-X
DO - 10.1016/0003-2670(91)87014-X
M3 - Article
AN - SCOPUS:0025833670
VL - 249
SP - 113
EP - 122
JO - Analytica chimica acta
JF - Analytica chimica acta
SN - 0003-2670
IS - 1
ER -