Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 070505 |
| Fachzeitschrift | Journal of biomedical optics |
| Jahrgang | 19 |
| Ausgabenummer | 7 |
| Publikationsstatus | Veröffentlicht - 28 Juli 2014 |
| Extern publiziert | Ja |
Abstract
In comparison to standard transfection methods, gold nanoparticle-mediated laser transfection has proven to be a versatile alternative. This is based on its minor influence on cell viability and its high efficiency, especially for the delivery of small molecules like small interfering RNA. However, in order to transfer it to routine usage, a safety aspect is of major concern: The avoidance of nanoparticle uptake by the cells is desired. The immobilization of the gold nanoparticles on cell culture surfaces can address this issue. In this study, we achieved this by silanization of the appropriate surfaces and the binding of gold nanoparticles to them. Comparable perforation efficiencies to the previous approaches of gold nanoparticle-mediated laser transfection with free gold nanoparticles are demonstrated. The uptake of the immobilized particles by the cells is unlikely. Consequently, these investigations offer the possibility of bringing gold nanoparticle-mediated laser transfection closer to routine usage
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Elektronische, optische und magnetische Materialien
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Physik und Astronomie (insg.)
- Atom- und Molekularphysik sowie Optik
- Ingenieurwesen (insg.)
- Biomedizintechnik
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in: Journal of biomedical optics, Jahrgang 19, Nr. 7, 070505, 28.07.2014.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Immobilization of gold nanoparticles on cell culture surfaces for safe and enhanced gold nanoparticle-mediated laser transfection
AU - Kalies, Stefan
AU - Heinemann, Dag
AU - Schomaker, Markus
AU - Gentemann, Lara
AU - Meyer, Heiko
AU - Ripken, Tammo
N1 - Funding Information: We would like to thank the German Research Foundation (DFG) and the REBIRTH Cluster of Excellence for funding.
PY - 2014/7/28
Y1 - 2014/7/28
N2 - In comparison to standard transfection methods, gold nanoparticle-mediated laser transfection has proven to be a versatile alternative. This is based on its minor influence on cell viability and its high efficiency, especially for the delivery of small molecules like small interfering RNA. However, in order to transfer it to routine usage, a safety aspect is of major concern: The avoidance of nanoparticle uptake by the cells is desired. The immobilization of the gold nanoparticles on cell culture surfaces can address this issue. In this study, we achieved this by silanization of the appropriate surfaces and the binding of gold nanoparticles to them. Comparable perforation efficiencies to the previous approaches of gold nanoparticle-mediated laser transfection with free gold nanoparticles are demonstrated. The uptake of the immobilized particles by the cells is unlikely. Consequently, these investigations offer the possibility of bringing gold nanoparticle-mediated laser transfection closer to routine usage
AB - In comparison to standard transfection methods, gold nanoparticle-mediated laser transfection has proven to be a versatile alternative. This is based on its minor influence on cell viability and its high efficiency, especially for the delivery of small molecules like small interfering RNA. However, in order to transfer it to routine usage, a safety aspect is of major concern: The avoidance of nanoparticle uptake by the cells is desired. The immobilization of the gold nanoparticles on cell culture surfaces can address this issue. In this study, we achieved this by silanization of the appropriate surfaces and the binding of gold nanoparticles to them. Comparable perforation efficiencies to the previous approaches of gold nanoparticle-mediated laser transfection with free gold nanoparticles are demonstrated. The uptake of the immobilized particles by the cells is unlikely. Consequently, these investigations offer the possibility of bringing gold nanoparticle-mediated laser transfection closer to routine usage
KW - cell manipulation
KW - gold nanoparticles
KW - laser transfection
KW - plasmonics
UR - http://www.scopus.com/inward/record.url?scp=84924502861&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.19.7.070505
DO - 10.1117/1.JBO.19.7.070505
M3 - Article
C2 - 25069006
AN - SCOPUS:84924502861
VL - 19
JO - Journal of biomedical optics
JF - Journal of biomedical optics
SN - 1083-3668
IS - 7
M1 - 070505
ER -