TY - JOUR

T1 - Immobilization and Application of Fatty Acid Photodecarboxylase in Deep Eutectic Solvents

AU - Chanquia, Santiago Nahuel

AU - Benfeldt, Frederik Vig

AU - Petrovai, Noémi

AU - Santner, Paul

AU - Hollmann, Frank

AU - Eser, Bekir Engin

AU - Kara, Selin

N1 - Funding Information: The authors thank the Novo Nordisk Foundation, Light‐BioFuels project, grant No. NNF19OC0057522. The authors are grateful to Dr. Karim Engelmark Cassimjee (EnginZyme A.B., Sweden) for providing free immobilization carrier samples.

PY - 2022/12/5

Y1 - 2022/12/5

N2 - Since its discovery in 2017, the fatty acid decarboxylase (FAP) photoenzyme has been the focus of extensive research, given its ability to convert fatty acids into alka(e)nes using merely visible blue light. Unfortunately, there are still some drawbacks that limit the applicability of this biocatalyst, such as poor solubility of the substrates in aqueous media, poor photostability, and the impossibility of reusing the catalyst for several cycles. In this work, we demonstrate the use of FAP in non-conventional media as a free enzyme and an immobilized preparation. Namely, its applicability in deep eutectic solvents (DESs) and a proof-of-concept immobilization using a commercial His-tag selective carrier, a thorough study of reaction and immobilization conditions in each case, as well as reusability studies are shown. We observed an almost complete selectivity of the enzyme towards C18 decarboxylation over C16 when used in a DES, with a product analytical yield up to 81 % when using whole cells. Furthermore, when applying the immobilized enzyme in DES, we obtained yields >10-fold higher than the ones obtained in aqueous media.

AB - Since its discovery in 2017, the fatty acid decarboxylase (FAP) photoenzyme has been the focus of extensive research, given its ability to convert fatty acids into alka(e)nes using merely visible blue light. Unfortunately, there are still some drawbacks that limit the applicability of this biocatalyst, such as poor solubility of the substrates in aqueous media, poor photostability, and the impossibility of reusing the catalyst for several cycles. In this work, we demonstrate the use of FAP in non-conventional media as a free enzyme and an immobilized preparation. Namely, its applicability in deep eutectic solvents (DESs) and a proof-of-concept immobilization using a commercial His-tag selective carrier, a thorough study of reaction and immobilization conditions in each case, as well as reusability studies are shown. We observed an almost complete selectivity of the enzyme towards C18 decarboxylation over C16 when used in a DES, with a product analytical yield up to 81 % when using whole cells. Furthermore, when applying the immobilized enzyme in DES, we obtained yields >10-fold higher than the ones obtained in aqueous media.

KW - deep eutectic solvents

KW - drop-in biofuels

KW - fatty acid

KW - immobilization

KW - photobiocatalysis

UR - http://www.scopus.com/inward/record.url?scp=85141784626&partnerID=8YFLogxK

U2 - 10.1002/cbic.202200482

DO - 10.1002/cbic.202200482

M3 - Article

VL - 23

JO - CHEMBIOCHEM

JF - CHEMBIOCHEM

SN - 1439-4227

IS - 23

M1 - e202200482

ER -